纳豆激酶是枯草芽孢杆菌发酵过程产生的一种碱性蛋白酶，具有较强的溶栓作用。与其他溶栓剂相比，纳豆激酶易被吸收，作用迅速，安全性好，因此是有待开发的新型溶栓剂。概述了纳豆激酶的理化性质和作用机制，着重介绍了纳豆激酶的几种常见的分离纯化方法，包括柱层析法、超滤法、萃取法和亲和颗粒法，以及集成化分离纯化技术，并对纳豆激酶分离纯化的新方法进行了展望，以促进纳豆激酶的深入开发和应用。

Nattokinase is a kind of serine protease produced by the fermentation of Bacillus subtilis, which has strong thrombolytic effect.Compared with other thrombolytic agents, nattokinase is easy to be absorbed and has a rapid effect and good safety. Therefore, it is a new thrombolytic agent to be developed.The mechanism and physicochemical properties of nattokinase were summarized,Several methods of isolation and purification of nattokinase were introduced,Including column chromatography, ultrafiltration, extraction and affinity particle adsorption, as well as integrated separation and purification technology,The new method of isolation and purification of nattokinase was prospected to improve the development and application of Nattokinase.