通过PCR技术从黑曲霉（Aspergillus  niger）基因组扩增获得一个全长1790 bp的α-L-阿拉伯呋喃糖苷酶基因，基因含有一个50 bp的内含子序列，其对应的蛋白质序列含有5个O糖基化位点和9个N糖基化位点，N端含有18个氨基酸的信号肽序列。将目的基因与表达载体pPICZαA连接并在毕赤酵母X-33诱导表达，获得重组α-L-阿拉伯呋喃糖苷酶。重组酶的相对分子质量为70 kDa，最适反应pH和温度分别为5.5和50 ℃；金属离子 Cu2+、Zn2+和Fe2+对重组酶的酶活有抑制作用，Fe3+对重组酶的酶活有促进作用；以4-Nitrophenyl α-L-arabinofuranoside为底物测得酶的Km值和Vmax值分别为0.78 mM和2.57 μmol/min/mg。在大麦麦芽协定糖化的初始阶段添加31.2 mU/g重组酶，麦汁的过滤速度提高了12.8%。以大麦麦芽阿拉伯木聚糖为底物，α-L-阿拉伯呋喃糖苷酶与木聚糖酶有较好的协同作用。

The arabidofuranosidase gene was amplified from theAspergillus nigergenome by PCR. The gene length was 1790 bp containing a intron sequence with 50 bp. The corresponding protein sequence contains 9 N-glycosylation sites and 5 O-glycosylationsites, the N-terminal contains 18 amino acids of the signal peptide. Arabidofuranosidase gene was ligated with the expression vector pPICZαA and induced inPichia pastorX-33. The recombinant enzyme was purified by Ni column affinity chromatography. The molecular size of the recombinant enzyme is 70 kDa, and the optimum reaction temperature and optimum pH of the recombinant enzyme were 50 ℃ and 5.5 respectively. Cu2+, Zn2+and Fe2+had inhibitory effect on the activity of Arabidofuranosidase, Fe3+promoted the enzyme activity of arabidofuranosidase. TheKmandVmaxvalues of the enzymes were 0.78 mM and 2.57 μmol/min/mg respectively, using 4-Nitrophenyl α-L-arabinofuranoside as the substrate. Recombinant α-L-arabinofuranosidase was added at the initial stage of mashing with an amount of 31.2 mU/g, wort filtration rate was increased by 12.8%. α-L-arabinofuranosidase and xylanase have synergistic effect with barley malt arabinoxylans as substrate.