黄芪对香菇菌丝体及子实体中成分的影响
网络出版日期: 2018-03-16
基金资助
“白灵菇新品种选育及配套技术研究与应用”(FT2014-03-13);山西省农业科学院特色农业攻关项目“黄芪功能性食用菌的开发研究与栽培应用”。
Effects of Astragalus on components of mycelia and fruiting body of Lentinus edodes
Online published: 2018-03-16
张劲松 , 牛宇 , 张丽珍 , 等 . 黄芪对香菇菌丝体及子实体中成分的影响[J]. 食品与发酵工业, 2018 , 44(2) : 113 . DOI: 10.13995/j.cnki.11-1802/ts.015735
The effects of Astragalus on the content of active ingredients of mycelia and fruiting bodies of Lentinus edodes were studied. The contents of total polysaccharides, total phenolic acids, total flavonoids and total saponins were determined by ultraviolet spectrophotometry. The content of calycosin-7-O-β-D-glucoside and fomononetin-7-O-glucoside were determined by high performance liquid chromatography. The results showed that the contents of total polysaccharides, total phenolic acids, total flavonoids, total saponins, calycosin-7-O-β-D-
glucoside and fomononetin-7-O-glucoside in Astragalus waste were 46.71 mg/g, 2.62 mg/g, 10.24 mg/g, 10.03 mg/g, 13.56 μg/g, 3.78 μg/g. Compared with Astragalus, the multiple of the components was 3.91, 1.89, 1.77, 2.05, 5.21, 5.24, respectively. The aqueous extract of Astragalus can inhibit the growth of mycelia of Lentinus edodes. Inhibition rate increased with the higher concentration of aqueous extract of Astragalus. When the maximum concentration of aqueous extract of Astragalus membranaceus was 0.5 g/100 mL, Lentinus edodes mycelium’s growth is not significantly inhibited. In Lentinus edodes mycelium which is added 0.5 g/100mL of aqueous extract of Astragalus, the contents of total polysaccharides, total phenols, total saponins and total saponins were 52.52 mg/g, 0.97 mg/g, 0.35 mg/g, 11.11 mg/g, the conversion rates were 1.08 % , 2.20 %, 0.17 % and 13.56 %. In Lentinus edodes fruiting bodies which is added Astragalus waste, the contents of polysaccharides, total phenolic acids, total flavonoids and total saponins were 60.48 mg/g, 1.16 mg/g, 0.68 mg/g, 3.56 mg/g, the conversion rates were 29.43 %, 6.26 %, 0.63 % and 9.09 % respectively. In the experimental group, the mycelium and the fruiting bodies of Lentinus edodes were found calycosin-7-O-β-D-glucoside, the contents were 1.09 μg/g and 0.86 μg/g respectively./
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