一株毛色二孢菌发酵产茉莉酸

董文华 , 郑璞 , 陈鹏程 , 等

doi:10.13995/j.cnki.11-1802/ts.015589

食品与发酵工业 >

2018 , Vol. 44 >Issue 3: 22

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.015589

一株毛色二孢菌发酵产茉莉酸

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(江南大学生物工程学院，工业生物技术教育部重点实验室，江苏无锡，214122)

硕士研究生

网络出版日期: 2018-03-26

基金资助

国家自然科学基金(21604032)；“十二五”农村领域国家科技计划课题(2015BAD15B04);中央高校基本科研业务费专项资金资助(JUSRP116031)；江南大学工业生物技术教育部重点实验室开放课题(KLIB-KF201501)

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Production of jasmonic acid by a strain Lasiodiplodia iranensis

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(The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122,China)

Online published: 2018-03-26

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摘要

从实验室保存和自然界分离到的100多株霉菌中筛选到1株高产茉莉酸的毛色二孢菌Lasiodiplodiairanensis DWH-2。优化得到发酵培养基组分为：蔗糖50 g/L，NaNO3 4.5 g/L，KH2PO4 2.0 g/L，KCl 0.4 g/L，MgSO4·7H2O 6 g/L，FeSO4· 7H2O 0.9 g/L，玉米浆1.5 g/L，微量元素10 ml/L，大豆油10 g/L；发酵初始pH 5.5，装液量100 mL/500 mL，培养温度28 ℃。在此条件下，发酵7 d，茉莉酸产量可达1 543 mg/L。

关键词： 茉莉酸; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; Lasiodiplodia iranensis; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; 发酵培养优化

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董文华 , 郑璞 , 陈鹏程 , 等 . 一株毛色二孢菌发酵产茉莉酸[J]. 食品与发酵工业, 2018 , 44(3) : 22 . DOI: 10.13995/j.cnki.11-1802/ts.015589

Abstract

A strain which could produce a high concentration of jasmonic acid was screened from more than 100 molds in nature and preserved in the laboratory. It was named as Lasiodiplodia iranensis DWH-2. Optimized fermentation medium composition contained sucrose 50 g/L, NaNO3 4.5 g/L, KH2PO4 2 g/L, KCl 0.4 g/L, MgSO4·7H2O 6 g/L, FeSO4·7H2O 0.9 g/L, corn pulp 1.5g/L, trace element 10 ml/L, soybean oil 10g/L, and its initial pH value was 5.5. The culture media volume and culture temperature was optimized to 100 mL/500 mL and 28 ℃, respectively. Under these conditions, the yield of jasmonic acid could reach 1.543 g/L after 7 days.

Key words： jasmonic acid; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; Lasiodiplodia iranensis; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; fermentation culture optimization

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