动植物蛋白源协同增强wistar大鼠抗疲劳能力

任广旭 , 伊素芹 , 张鸿儒 , 等

doi:10.13995/j.cnki.11-1802/ts.014787

食品与发酵工业 >

2018 , Vol. 44 >Issue 3: 65

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.014787

动植物蛋白源协同增强wistar大鼠抗疲劳能力

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1(农业部食物与营养发展研究所，北京，100081) 2(中国农业科学院双蛋白工程研究中心，北京，100081)
3(农业部农产品质量与营养功能风险评估试验室，北京，100081)

博士,助理研究员

网络出版日期: 2018-03-26

基金资助

国家自然科学基金(81703221)；中国农业科学院创新工程项目 (CAAS-ASTIP320173IFND);农业部食物与营养发展研究所；基本科研业务费专项 (NO.1610422016003 and NO.1610422017007)

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Effects of dual protein on anti-fatigue capacity during endurance excise of wister rats

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1(Institute of Food and Nutrition Development，Ministry of Agriculture, Beijing 100081, China)
2(Engineering Research Center of CAAS for Dual Protein, Beijing 100081, China)
3(Risk Assessment Laboratory of Quality and Nutritional Function of Agricultural Product(Beijing)Ministry of
Agriculture，Beijing 100081，China)

Online published: 2018-03-26

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摘要

通过6周蛋白干预，探讨运动后摄入“大豆+牛乳”动植物蛋源对运动性疲劳的影响。SPF级wistar系雄性大鼠18只随机分为3组：大豆蛋白组(n=6)、乳清蛋白组(n=6)和双蛋白组(n=6)。采用大鼠尾部负重静水游泳方式，建立一次性力竭运动模型。每周5 d进行负重游泳训练，训练后各组补充相应蛋白质，第7周记录每只大鼠力竭时间，并在蛋白干预后每隔30 min采集尾静脉血，检测肌酸激酶(CK)和乳酸脱氢酶(LDH)活性。结果表明,通过6周的蛋白饲喂，大鼠在运动后补充双蛋白可使一次性力竭平均时间达到135.8 s，显著高于乳清蛋白组(平均力竭时间88 s)和大豆分离蛋白组(平均力竭时间99.8 s)。双蛋白组在负重游泳后第30 min乳酸脱氢酶活性显著高于乳清蛋白组和大豆分离蛋白组，第60 min就恢复到与其他2组一致水平。因此,运动后及时补充“大豆+乳清”双蛋白具有抗疲劳作用，提高大鼠运动能力。

关键词： 大豆蛋白; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; 乳清蛋白; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; 抗疲劳; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; 运动训练

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任广旭 , 伊素芹 , 张鸿儒 , 等 . 动植物蛋白源协同增强wistar大鼠抗疲劳能力[J]. 食品与发酵工业, 2018 , 44(3) : 65 . DOI: 10.13995/j.cnki.11-1802/ts.014787

Abstract

To investigate the effects of dual protein on exercise fatigue, 18 healthy and SPF grade wistar male rats were randomly divided into 3 groups: soy protein(n=6), whey protein(n=6), and dual protein groups(n=6). We used weight loaded force swimming test to evaluate anti-fatigue capacity. Tail venous blood was collected to detect the activity of creatine kinase and lactate dehydrogenase at certain time period. After six weeks of feeding, mean exhaustive time of the rats fed with dual protein can reach to135.8 s which was significantly higher than the whey protein group (88 s) and soybean protein group (99.8 s). Lactate dehydrogenase activity of dual protein group reached to pick value in 30 min and went back to the same level as others in 60 min. Thus, supplement of dual protein after exercise can help recover anti-fatigue quickly and improve the endurance capacity of wistar rats.

Key words： soybean protein; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; whey protein; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; antifatigue; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; sports training

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