苯乳酸生产菌的筛选及鉴定

黄国昌 , 顾斌涛 , 黄朝 , 等

doi:10.13995/j.cnki.11-1802/ts.016064

食品与发酵工业 >

2018 , Vol. 44 >Issue 3: 97

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.016064

苯乳酸生产菌的筛选及鉴定

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(江西省科学院微生物研究所，江西南昌，330096)

硕士，副研究员

网络出版日期: 2018-03-26

基金资助

江西省科技支撑计划重点项目(20151BBG70002)；江西省科学院重点科研项目(2017-YZD2-01)

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Screening and identification of phenyllactic acid-producing bacteria

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(Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang 330096,China)

Online published: 2018-03-26

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摘要

苯乳酸是一种具有宽广抑菌谱的新型生物保鲜剂，为筛选得到苯乳酸高产菌株，采用改良的碳酸钙溶解圈法和抑菌圈法，从自然环境中采集的50个样品中分离筛选得到2株高产苯乳酸的乳酸菌，分别命名为BLPC002和SCPC008，并对其进行了初步鉴定。结果显示，BLPC002和SCPC008在以苯丙氨酸为底物的条件下，摇瓶发酵苯乳酸产量分别为358 mg/L和333 mg/L，较改良前不到100 mg/L有明显的提高，说明改良后的筛选方法更加高效、准确。进一步通过对菌株形态和16S rDNA序列分析初步鉴定，均为植物乳杆菌。

关键词： 苯乳酸; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; 乳酸菌; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; 筛选

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黄国昌 , 顾斌涛 , 黄朝 , 等 . 苯乳酸生产菌的筛选及鉴定[J]. 食品与发酵工业, 2018 , 44(3) : 97 . DOI: 10.13995/j.cnki.11-1802/ts.016064

Abstract

Phenyllactic acid is a new biological preservative with broad antibacterial spectrum. In order to obtain high yield lactic acid bacteria, 2 lactic acid bacteria strains with high phenyllactic acid yield were isolated from 50 samples in different environment with the modified methods of dissolving calcium ring and bacteriostatic circle, which were named respectively as BLPC002 and SCPC008, and the strains were identified. The results showed that the yield of phenyllactic acid in shake flask fermentation with phenylalanine as substrate was 358 mg/L and 333 mg/L, respectively. The yields of phenyllactic acid were evidently improved compared with less than 100 mg/L before modification. It indicated that the modified method was more efficient and accurate. The strains were identified as Lactobacillus plantarum by morphological analysis and 16S rDNA sequencing.

Key words： phenyllacticacid(PLA); color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; lactic acid bacteria; color:#2453B3; font-family:"; ????"; font-size:12px; font-style:normal; font-weight:700; line-height:18px; text-decoration:none; ">; screening

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