粪肠球菌Enterococcus faecalis EC-12的万古霉素耐药基因检测

刘洋 , 张欣 , 陈建国 , 等

doi:10.13995/j.cnki.11-1802/ts.016165

食品与发酵工业 >

2018 , Vol. 44 >Issue 3: 226

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.016165

粪肠球菌Enterococcus faecalis EC-12的万古霉素耐药基因检测

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1(中国食品发酵工业研究院，中国工业微生物菌种保藏管理中心，北京，100015)
2(日本 Combi株式会社，埼玉，3380832)

博士，高级工程师

网络出版日期: 2018-03-26

基金资助

国家重点研发计划资助(2016YFD0400502)

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Detection of Vancomycin-resistant genes of strain Enterococcus faecalis EC-12

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1 (China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries,
Beijing 100015, China) 2(Japan Combi Corporation, Saitama 3380832)

Online published: 2018-03-26

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摘要

对粪肠球菌Enterococcus faecalis EC-12的万古霉素耐药基因进行了筛查。通过PCR方法，以7种典型肠球菌万古霉素耐药基因(vanA、vanB、vanC1、vanC2 /C3、vanD、vanE和vanG)进行特异性扩增，结果均为阴性。采用PCR方法检测万古霉素耐药肠球菌简便、高效、准确。

关键词： ; font-family:SimSun; color:#000000; font-size:16px; font-weight:normal; ">粪肠球菌; 万古霉素; 耐药基因; PCR

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刘洋 , 张欣 , 陈建国 , 等 . 粪肠球菌Enterococcus faecalis EC-12的万古霉素耐药基因检测[J]. 食品与发酵工业, 2018 , 44(3) : 226 . DOI: 10.13995/j.cnki.11-1802/ts.016165

Abstract

Vancomycin resistant genes of strain Enterococcus faecalis EC-12 were screened in this study. PCR specific for seven typical genes vanA, vanB, vanC1, vanC2/C3, vanD, vane and vanG were carried out. Results showed that all of them were negative in strain EC-12. This specific PCR method could be used to detect vancomycin-resistant Enterococcus sp.

Key words： ; font-family:SimSun; color:#000000; font-size:16px; font-weight:normal; ">Enterococcus; font-family:SimSun; color:#000000; font-size:16px; font-weight:normal; "> ; ; font-family:SimSun; color:#000000; font-size:16px; font-weight:normal; ">faecalis; color:#000000; font-size:16px; font-weight:normal; ">; ; font-family:SimSun; color:#000000; font-size:16px; font-weight:normal; "> Vancomycin; resistant gene; specific PCR

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