将一种源于假单胞菌的目的基因克隆到表达载体pET-22b(+)，构建重组质粒pET-22b(+)-tao，并转入到大肠杆菌E.coli BL21(DE3)中进行诱导表达。获得的E.coli BL21(DE3)(tao)于37℃培养70～90 min后，加入终浓度为0.4 mmol/L 的异丙荃硫代半乳糖苷(isopropyl β-D-thiogalactoside, IPTG)，20 ℃诱导6 h，黄素单加氧酶(trans-anethole oxygenase, TAO)酶活可达35 U/g。在pH值为8、反应温度为30 ℃的条件下，重组菌全细胞转化黄樟油素生成洋茉莉醛的质量浓度可达9.15 g/L。反应液经乙酸乙酯萃取、浓缩及干燥处理后得到产物晶体，红外色谱以及核磁共振分析证实其为洋茉莉醛，高效液相色谱测定其纯度为97%以上。

A recombinant plasmid pET-22b (+)-tao was successfully constructed by inserting the TAO oxidase gene derived from Pseudomonas to the plasmid vector pET-22b (+). The recombinant plasmid was transformed to the E. coli BL21 (DE3) to get E.coli BL21 (DE3)(tao). After recombinant the strain was cultured at 37 ℃ for 70-90 min, IPTG was added with a final concentration of 0.4 mmol/L. After induction for 6 h at 20 ℃, the TAO oxidase activity could reach 35 U/g. The concentration of heliotropin could reach 9.15 g/L after reaction at pH 8 and 30 ℃. Product was obtained in crystal after extraction by ethyl acetate and drying. IR and 1H-NMR verified that the crystal was heliotropin. HPLC showed that the purity was higher than 97%.