以黑豆皮为实验材料，用乙醇浸提法对黑豆皮中的花色苷进行提取，用大孔吸附树脂对花色苷进行纯化，经冷冻干燥得到黑豆皮花色苷粗品。利用中压制备色谱对花色苷组分进行分离，通过质谱分析鉴定经中压制备色谱分离后的花色苷组分。结果表明：黑豆皮花色苷粗品中的总花色苷含量为26.9%，经中压制备色谱对花色苷粗品进行分离后的2峰中矢车菊素-3-葡萄糖苷纯度达到91.46%。黑豆皮中的主要花色苷为天竺葵素-3-O-芸香糖苷、芍药色素-3-O-葡萄糖苷、矢车菊素-3-O-葡萄糖苷和锦葵素-3-葡萄糖苷-4-乙醛。

The anthocyanins from black soybean peel were extracted with ethanol extraction method. The anthocyanins were purified by macroporous resin. The crude product was obtained after freeze-drying. The anthocyanin fraction was isolated by medium-pressure liquid chromatography and identified by mass spectrometry. The results showed as followes: the total anthocyanin content in the crude product of black bean peel was 26.9%; the purity of cyanidin-3-glucoside in the 2 peaks after separation of black soybean peel crude anthocyanins by medium-pressure liquid chromatography reached 91.46%. The main anthocyanins in the black bean peel were Pelargonidin-3-O-rutinosie, Peonidin-3-O- glucoside, Cyanidin-3-O-glucoside and Vitisin B.