微生物共培养降解β-氯氰菊酯的适宜条件

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  • 1(四川大学 轻纺与食品学院,四川 成都,610065) 2(旌阳区食品药品监督管理局,四川 德阳,618000) 3(四川师范大学 生命科学学院,四川 成都,610101)

网络出版日期: 2018-08-30

基金资助

国家自然科学基金项目(31371775)

Appropriate conditions of β-cypermethrin degradation by a co-culture of microorganism

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  • 1(College of Light Industry and Food, Sichuan University, Chengdu 610065,China) 2(Jingyang Food and Drug Administration, Deyang 618000,China) 3(College of Life Science, Sichuan Normal University, Chengdu 610101,China)

Online published: 2018-08-30

摘要

从长期喷施拟除虫菊酯类农药的菜园土壤中筛选到1株对β-氯氰菊酯具有降解能力的放线菌。生理生化及16S rDNA序列鉴定结果显示,该菌株归属于链霉菌(Streptomyces sp. K-1)。将该菌株与前期筛选的对β-氯氰菊酯具有较强降解能力的地衣芽孢杆菌B-1(Bacillus licheniformis B-1)共同培养,确定了其降解β-氯氰菊酯的适宜条件。当两种培养基的混合比例为1∶1(GSM∶LB, v/v)、菌株K-1和菌株B-1的接种比例为2∶1(v/v)、接种方式为接种菌株K-1培养24 h后再接种菌株B-1、35 ℃振荡(150 r/min)培养72 h时,对含量为50 μg/mL β-氯氰菊酯的降解率可达88.3%。

本文引用格式

苏宏南, 陈切希, 赵甲元, 等. . 微生物共培养降解β-氯氰菊酯的适宜条件[J]. 食品与发酵工业, 2018 , 44(7) : 8 -12 . DOI: 10.13995/j.cnki.11-1802/ts.016440

Abstract

An actinomyce, which could degrade β-cypermethrin, was isolated from kale yard soil sprayed pyrethroid pesticides for a long time. According to physic-biochemical characteristics and 16S rDNA sequence, the strain was classified as Streptomyces sp. K-1. Strain K-1 and Bacillus licheniformis B-1 with high degradation capacity on β-cypermethrin were co-cultured, and the appropriate degradation conditions of β-cypermethrin in the system were selected. Suitable conditions were as follows: mixed medium ratio (GSM∶LB, v/v) of 1∶1, inoculation ratio (K-1∶B-1, v/v) of 2∶1, inoculating strain B-1 after inoculation of strain K-1 for 24 hours, and 35 ℃ on a rotary shaker at 150 r/min. Under these conditions, 88.3% β-Cypermethrin (50 μg/mL) could be removed within 72 hours.
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