从长期喷施拟除虫菊酯类农药的菜园土壤中筛选到1株对β-氯氰菊酯具有降解能力的放线菌。生理生化及16S rDNA序列鉴定结果显示,该菌株归属于链霉菌(Streptomyces sp. K-1)。将该菌株与前期筛选的对β-氯氰菊酯具有较强降解能力的地衣芽孢杆菌B-1(Bacillus licheniformis B-1)共同培养,确定了其降解β-氯氰菊酯的适宜条件。当两种培养基的混合比例为1∶1(GSM∶LB, v/v)、菌株K-1和菌株B-1的接种比例为2∶1(v/v)、接种方式为接种菌株K-1培养24 h后再接种菌株B-1、35 ℃振荡(150 r/min)培养72 h时,对含量为50 μg/mL β-氯氰菊酯的降解率可达88.3%。
An actinomyce, which could degrade β-cypermethrin, was isolated from kale yard soil sprayed pyrethroid pesticides for a long time. According to physic-biochemical characteristics and 16S rDNA sequence, the strain was classified as Streptomyces sp. K-1. Strain K-1 and Bacillus licheniformis B-1 with high degradation capacity on β-cypermethrin were co-cultured, and the appropriate degradation conditions of β-cypermethrin in the system were selected. Suitable conditions were as follows: mixed medium ratio (GSM∶LB, v/v) of 1∶1, inoculation ratio (K-1∶B-1, v/v) of 2∶1, inoculating strain B-1 after inoculation of strain K-1 for 24 hours, and 35 ℃ on a rotary shaker at 150 r/min. Under these conditions, 88.3% β-Cypermethrin (50 μg/mL) could be removed within 72 hours.