α-L-鼠李糖苷酶高密度发酵及其固定化

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  • (集美大学 食品与生物工程学院,福建 厦门,361021)

网络出版日期: 2018-08-30

基金资助

国家自然基金(31371751)

High density fermentation of α-L-rhamnosidase and enzyme immobilization

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  • (College of Food and Biological Engineering, Jimei University, Xiamen 361021, China)

Online published: 2018-08-30

摘要

在5 L发酵罐中对α-L-鼠李糖苷酶进行小试发酵,制备酶液;以聚乙烯醇-海藻酸钠为包埋载体,以戊二醛为交联剂对该酶进行固定化。经5 L罐高密度发酵96 h,α-L-鼠李糖苷酶酶活为2 766 U/g,生物量为228 g/L。经过固定化后,α-L-鼠李糖苷酶的酶活为20 U/g,酶活回收率为32.88%,固定化α-L-鼠李糖苷酶的最适反应温度为35~50 ℃,最适反应pH值为4.0,该固定化酶在连续使用6次后酶活仍然保留98.22%。高密度发酵能显著提高酶的产量,同时固定化技术提高了酶的重复利用率,为该酶利用芦丁酶法转化制备异槲皮苷提供了依据。

本文引用格式

刘嘉男, 巩建业, 高庭, 等. . α-L-鼠李糖苷酶高密度发酵及其固定化[J]. 食品与发酵工业, 2018 , 44(7) : 44 -48 . DOI: 10.13995/j.cnki.11-1802/ts.016143

Abstract

α-L-rhamnosidase produced by high density fermentation was immobilized into matrix consisting of polyvinyl alcohol and sodium alginate with glutaraldehyde as crosslinker. In the 5 L bioreactor, the activity of immobilized enzyme achieved 2 766 U/g and its biomass reached 228 g /L after 96 h cultivation. The enzyme activity recovery of 32.88% was obtained and the activity of immobilized enzyme was 20 U/g. The optimal temperature was 35-50 ℃ and the optimal reaction pH at about 4.0 for immobilized enzyme. The residual enzyme activity could retain more than 98.22% after 6 batches of recycling use. These results suggested that the high density fermentation could effectively enhance enzyme production, and the immobilization technology could improve enzyme recycling rate, which would offer a basis for large-scale production of isoquercitrin.
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