通过96/6孔板培养法进行生物膜体外模型建立，比浊法测定生物膜含量，结晶紫染色法观察生物膜形态，平板菌落计数法评价超声波对玻璃表面生物膜的去除作用效果，激光共聚焦显微镜观察超声波处理后生物膜脱落效果，研究阪崎肠杆菌生物膜的形成条件和超声波处理对其去除作用效果。结果表明，阪崎肠杆菌生物膜形成的最适温度37 ℃，最适pH 7.5，最适盐度为2.5%。CaCl2(0.50%)、MgCl2(1.50%)对阪崎肠杆菌生物膜形成具有明显促进作用。Cu2+(1.50% )、EDTA(2.5%)对阪崎肠杆菌生物膜形成具有抑制作用。超声波处理能够使玻璃表面上附着的阪崎肠杆菌生物膜脱落，超声波处理初始温度30 ℃，时间14 min条件下去除效果最好。激光共聚焦显微镜可直接观察到超声波处理后盖玻片上生物膜中阪崎肠杆菌活菌数量变化，可用于证实和评价超声处理对玻璃表面阪崎肠杆菌生物膜的去除效果。

The formation condition of Cronobacter sakazakii biofilm and its elimination by ultrasound treatment on glass surfaces were investigated through biofilm establishment in vitro model by 96/6 orifice culture method and turbidimetric determination of biofilm content. The morphology of biofilm was observed by crystalline violet staining. The ultrasonic shedding was evaluated by plate colony counting method and the elimination effect of ultrasonic treatment was observed by laser scanning confocal microscopy. The optimal temperature and pH for Cronobacter sakazakii formation was 37 ℃ and 7.5. NaCl (2.5%), CaCl2(0.50%) and MgCl2(1.50%) added in medium promoted biofilm formation obviously, Cu2+(1.50%) and EDTA(2.5%) added in medium inhibited bioflm formation. Cronobacter sakazakii biofilm attached to the glass surface was eliminated by ultrasonic treatment. The elimination effect was the best when initial temperature was 30 ℃ and time was 14 min. The change of living cells number on biofilm after ultrasound treatment was observed by laser scanning confocal microscopy to prove and evaluate the elimination effect of ultrasonic treatment.