采用大孔弱酸钠型阳离子交换树脂Amberlite IRC-50分离纯化发酵液中的ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)。通过对其动态上样条件、洗涤条件和洗脱条件的优化，确定出最佳离子交换工艺：上样pH值为7.0，上样流速5 BV/h；以去离子水作为洗涤剂，洗涤流速2 BV/h；以0.25 mol/L NaOH作为洗脱剂，洗脱流速2 BV/h。在此离子交换操作条件下，钠型Amberlite IRC-50工作交换容量为305.12 mg/g，解吸率在95%以上；ε-PL纯度由24.29%提升至75.26%，ε-PL回收率达到96.40%。

A macroporous and weak acid cation exchange resin, Na+-form Amberlite IRC-50, was employed to separate and purify ε-poly-L-lysine (ε-PL) from fermentation broth. Based on the optimization of uploading, wishing and elution parameters, the optimal dynamic ion exchange conditions were developed as following: the sample was uploaded at pH 7.0 with loading rate at 5 BV/h; deionized water was used for washing resin at 2 BV/h; 0.25 mol/L NaOH was used as eluent with elution rate at 2 BV/h. Under the above conditions, the working exchange capacity of the resin was reached by 305.12 mg/g, and the desorption rate was attained to more than 95%. Finally, the purity of ε-PL was enhanced from the initial 24.29% to 75.26%, and the ε-PL recovery rate was also reached by 96.40%.