液液萃取结合GC-O和GC-MS解析镇江香醋香气成分

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  • (教育部工业生物技术重点实验室,江南大学 生物工程学院,酿造微生物与应用酶学研究室,江苏 无锡,214122)

网络出版日期: 2018-09-06

基金资助

国家重点研发计划(National Key R&D Program, 2016YFD0400503)

Characterization of volatile aroma components in Zhenjiang aromatic vinegar by liquid-liquid extraction combined with GC-O and GC-MS

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  • ( Key Laboratory of Industrial Biotechnology,Ministry of Education,Laboratory of Brewing Microbiology and Applied Enzymology,School of Biotechnology,Jiangnan University,Wuxi 214122,China)

Online published: 2018-09-06

摘要

采用液液萃取(liquid-liquid extraction,LLE)结合溶剂辅助风味蒸发(solvent assisted flavor evaporation,SAFE)技术萃取镇江香醋中香气化合物,将萃取物分为中-碱性组分和酸性-水溶性组分,应用香气稀释萃取分析法(aroma extract dilution analysis,AEDA)共鉴定出60种香气物质。基于FD值,最重要香气物质是3-(甲硫基)丙醛、2,3,5,6-四甲基吡嗪、3-甲基丁酸和香兰素(FD=729)。此外,2,3-丁二酮、乙酸、糠醛、3-(甲硫基)-1-丙醇、愈创木酚、2-苯乙酸以及γ-壬内酯也具有较高FD值(FD=243),这些化合物是镇江香醋的重要香气物质。

本文引用格式

袁源, 范文来, 徐岩. . 液液萃取结合GC-O和GC-MS解析镇江香醋香气成分[J]. 食品与发酵工业, 2018 , 44(7) : 238 -242 . DOI: 10.13995/j.cnki.11-1802/ts.016733

Abstract

The aroma components in Zhenjiang aromatic vinegarwere concentrated by liquid-liquid extraction (LLE) combined with solvent assisted flavor evaporation (SAFE), and further separated into neutral-basic and acidic-water soluble fractions. The total 60 aroma-active compounds in Zhenjiang aromatic vinegar were detected and identified by GC-O coupled with GC-MS. Aroma extract dilution analysis (AEDA) was employed to identify the most important aroma components. Of these, 2,3,5,6-tetramethylpyrazine, 3-(methylthio) propanal, 3-methybutanoic acid, and vanillin could be the most important aroma components in Zhenjiang aromatic vinegar with the highest FD value (FD=729). In addition, 2,3-butanedione, acetic acid, furfural, 3-(methylthio)-1-propanol, guaiacol, 2-phenylacetic acid, and γ-nonalactonecould be important (FD=243), which also made contribution to the aroma of Zhenjiang aromatic vinegar.
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