葡萄糖氧化酶是一种能够专一性催化β-D-葡萄糖氧化生成葡萄糖酸和过氧化氢的需氧脱氢酶,在食品、医药、化工、纺织、生物技术等领域具有重大应用价值。微生物是产葡萄糖氧化酶的主要来源,具有生长繁殖快、产酶效率高、纯化过程简单、适合商业化生产等特点。该文从产葡萄糖氧化酶的微生物来源、产酶菌株的基因工程改造及葡萄糖氧化酶的应用等方面对微生物葡萄糖氧化酶进行综述,为产葡萄糖氧化酶微生物的开发及基因工程技术在菌种选育上的应用提供理论参考。
Glucose oxidase is an aerobic dehydrogenase that specifically catalyzes the oxidation of β-D-glucose to produce gluconic acid and hydrogen peroxide. It has great application value in food, medicine, chemical industry, textile, biotechnology and other fields. Microbes are the main source of glucose oxidase, which have the advantages of fast growth and reproduction, high efficiency of enzyme production, and simple purification proces. This paper reviews the microbial sources of glucose oxidase, the genetic engineering transformation of enzyme producing strains and the application of glucose oxidase. It provides a theoretical reference for the development of glucose oxidase-producing microorganism and the application of genetic engineering technology in strain selection.