为了延缓丁酸梭菌芽孢形成，提高丁酸梭菌的生长效率，在20 L发酵罐水平研究了发酵基料添加鸟嘌呤(0.2 g/L)、Ile(0.5 g/L)对丁酸梭菌生长、酸代谢的影响。结果表明：添加鸟嘌呤或Ile的最大生物量分别达到5.31×108 CFU/mL和 4.65×108 CFU/mL，较对照(4.32×108 CFU/mL)分别提高了22.91%和7.64%；丁酸的菌体得率(Yp/x)分别较对照(0.37 g/g)减少了48.64%和40.54%。鸟嘌呤和Ile均显著降低了丁酸的生成效率、促进了细胞的生长、延缓了芽孢的形成。当发酵基料同时添加鸟嘌呤和Ile时，生物量达到5.4×108 CFU/mL，比对照提高了25%，为丁酸梭菌的工业化生产提供了新思路。

In order to delay the sporulation and improve the growth efficiency of Clostridium butyricum, the effects of guanine (0.2 g/L) and Ile (0.5 g/L) on the growth and acids metabolism of C. butyricum in 20 L bioreactor were investigated. The results showed that the maximum biomass with the addition of guanine or Ile reached 5.31×108 CFU/mL and 4.65×108 CFU/mL, respectively, which was increased by 22.91% and 7.64% compared with the control (4.32×108 CFU/mL). The coefficient of butyric acid to maximum biomass (Yp/x) was decreased by 48.64% and 40.54% compared with the control (0.37 g/g), respectively. Gunanine and/or Ile addition could reduce the specific butyrate productivity, enhance the growth efficiency and delay the sporulation of C. butyricum. The biomass reached 5.4×108 CFU/mL when guanine (0.2 g/L) and Ile (0.5 g/L) were simultaneously added to the fermentation medium, which was 25% higher than that of the control. This research provides new methods for industrial fermentation of C. butyricum.