豌豆肽对环磷酰胺致免疫抑制小鼠免疫功能的影响

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  • 1(北京大学 公共卫生学院,营养与食品卫生学系,北京,100191) 2(中国食品发酵工业研究院有限公司,北京市蛋白功能肽工程技术研究中心,北京,100015) 3(宁波御坊堂生物科技有限公司,浙江 宁波,315012) 4(食品安全毒理学研究与评价北京市重点实验室,北京,100191)

网络出版日期: 2018-09-14

基金资助

十三五国家重点研发计划(2016YFD0400604);北京市科委专项(北京市科委科技创新基地培育与发展工程专项)(Z161100005016030);国家自然科学基金(31671963)

Effects of pea peptide on immune function in immunosuppressed mice induced by cyclophosphamide

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  • 1(Department of Nutrition and Food Hygiene, Peking University School of Public Health, Beijing 100191, China) 2(China National Research Institute of Food and Fermentation Industries CO. LTD, Beijing Engineering Research Center of Protein and Functional Peptides, Beijing 100015, China) 3(Ningbo Yu Fang Tang Biological Science and Technology Co., Ltd., Ningbo 315012, China) 4(Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China)

Online published: 2018-09-14

摘要

研究豌豆肽对免疫抑制小鼠的免疫调节作用。腹腔注射环磷酰胺(cyclophosphamide,CTX)建立小鼠免疫抑制模型,并设置空白对照组,观察不同剂量豌豆肽[低剂量组0.4 g/(kg·bw)、中剂量组0.8 g/(kg·bw)、高剂量组1.6 g/(kg·bw)]干预15 d后对小鼠体重、免疫脏器重量及形态、白细胞计数、脾脏T淋巴细胞亚群、免疫球蛋白含量、细胞因子水平、骨髓有核细胞计数和DNA含量的影响。模型组小鼠脾脏和胸腺镜下形态学结构紊乱,外周血白细胞计数、CD3+、CD4+、CD8+ T淋巴细胞百分比、免疫球蛋白IgG和IgM含量、骨髓有核细胞浓度和DNA含量均显著低于对照组(p<0.01),细胞因子IFN-γ浓度明显低于对照组(p<0.05)。与模型组相比,豌豆肽组小鼠在以上指标有不同程度的改善。一定剂量的豌豆肽对CTX致免疫抑制小鼠的免疫功能具有一定的改善作用。

本文引用格式

张敏佳, 刘文颖, 贾福怀, 等 . 豌豆肽对环磷酰胺致免疫抑制小鼠免疫功能的影响[J]. 食品与发酵工业, 2018 , 44(8) : 135 -140 . DOI: 10.13995/j.cnki.11-1802/ts.015585

Abstract

The aim of this study was to investigate the immuno-regulatory effects of pea peptides on immunosuppressive mice. Mice injected with cyclophosphamide(CTX)was the model group and normal saline was the control group. Different dosages of pea peptide(0.4 g/(kg·bw),0.8 g/(kg·bw),1.6 g/(kg·bw)were given to low,middle and high groups for 15 days. The body weight, spleen, and thymus weight of mice,morphologic variations of immune organs,white blood cell count,T lymphocyte subsets of spleen,immunoglobulin content,cytokine levels, bone marrow nucleated cell count and DNA content were tested. The morphological changes of spleen and thymus were observed under microscope in the model group. The number of white blood cell,proportion of CD3+,CD4+,CD8+ T lymphocyte,concentration of immunoglobulin IgG and IgM, and nucleated cell counts and DNA density in bone marrow were significantly lower than the control group (p<0.01). The concentration of IFN-γ was significantly lower than that of the control group (p<0.05). Compared with the model group, pea peptide groups showed different degrees of improvement in the above indicators. Pea peptide can improve the immune function of immunosuppressive mice induced by CTX.
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