酵母静息细胞耦合原位分离技术连续转化2-苯乙醇

  • 黄筱萍 ,
  • 刘 兰 ,
  • 熊大维 ,
  • 等.
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  • (江西省科学院 微生物研究所,江西 南昌,330029)
硕士,研究员

收稿日期: 2018-05-15

  修回日期: 2018-05-29

  网络出版日期: 2018-11-23

基金资助

江西省重大研发项目(20181ACF60024);重点研发项目(20161BBE50098);江西省科学院产学研合作资金项目(2017-YCXF-09)

Continuous bioconversion of 2-phenylethanol by yeast resting cells coupled within situ product removal technique

  • HUANG Xiao-ping ,
  • LIU Lan ,
  • XIONG Da-wei ,
  • et al.
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  • (Institution of Microbiology, Jiangxi Academy of Sciences, Nanchang 330029, China)

Received date: 2018-05-15

  Revised date: 2018-05-29

  Online published: 2018-11-23

摘要

以酵母静息细胞作为酶载体,大孔吸附树脂为分离介质,微滤膜分离菌体,采用原位产物分离技术进行连续转化合成2-苯乙醇(2-phenylethanol,简称2-PE)。在5 L发酵罐中优化了转化条件,通过补料和控制产物浓度实现连续转化合成2-苯乙醇。单罐运行144 h,2-苯乙醇产量达24.06 g/L,摩尔转化率和平均转化速率分别为79.34%和0.166 g/(L·h)。双罐运行144 h,2-苯乙醇产量达29.86 g/L,比单罐产量提高24.1%;摩尔转化率和平均转化速率分别达81.83%和0.204 6 g/(L·h),静息细胞重复使用10批次,2-PE产量和平均转化速率没有明显下降。

本文引用格式

黄筱萍 , 刘 兰 , 熊大维 , 等. . 酵母静息细胞耦合原位分离技术连续转化2-苯乙醇[J]. 食品与发酵工业, 2018 , 44(10) : 20 -24 . DOI: 10.13995/j.cnki.11-1802/ts.017601

Abstract

Using the yeast resting cells and macroporous adsorptive resins respectively as enzyme carriers and separation medium, continuous transformation of 2-phenylethanol was carried out with the aid of in situ product removal techniques. Meanwhile, the strains were separated through microfiltration membrane. The transformation conditions were optimized in 5 L fermentor, and continuous bioconversion of 2-phenylethanol was fulfilled by feeding and controlling product concentration. The concentration of 2-phenylethanol was 24.06 g/L after running 144 h in single tank. The molar conversion rate was 79.34 %, and the average space-time yield was 0.166 g/(L·h). After running 144 h in double tanks, the concentration of 2-phenylethanol reached 29.86 g/L, which was higher than single tank production by 24.1%. The molar conversion rate and the average space-time yield were up to 81.83 % and 0.204 6 g/(L·h), respectively. After repeated use of resting cells for 10 times, the production and average conversion rate of 2-PE did not decrease obviously.
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