以酵母静息细胞作为酶载体，大孔吸附树脂为分离介质，微滤膜分离菌体，采用原位产物分离技术进行连续转化合成2-苯乙醇(2-phenylethanol，简称2-PE)。在5 L发酵罐中优化了转化条件，通过补料和控制产物浓度实现连续转化合成2-苯乙醇。单罐运行144 h，2-苯乙醇产量达24.06 g/L，摩尔转化率和平均转化速率分别为79.34%和0.166 g/(L·h)。双罐运行144 h，2-苯乙醇产量达29.86 g/L，比单罐产量提高24.1%；摩尔转化率和平均转化速率分别达81.83%和0.204 6 g/(L·h)，静息细胞重复使用10批次，2-PE产量和平均转化速率没有明显下降。

Using the yeast resting cells and macroporous adsorptive resins respectively as enzyme carriers and separation medium, continuous transformation of 2-phenylethanol was carried out with the aid of in situ product removal techniques. Meanwhile, the strains were separated through microfiltration membrane. The transformation conditions were optimized in 5 L fermentor, and continuous bioconversion of 2-phenylethanol was fulfilled by feeding and controlling product concentration. The concentration of 2-phenylethanol was 24.06 g/L after running 144 h in single tank. The molar conversion rate was 79.34 %, and the average space-time yield was 0.166 g/(L·h). After running 144 h in double tanks, the concentration of 2-phenylethanol reached 29.86 g/L, which was higher than single tank production by 24.1%. The molar conversion rate and the average space-time yield were up to 81.83 % and 0.204 6 g/(L·h), respectively. After repeated use of resting cells for 10 times, the production and average conversion rate of 2-PE did not decrease obviously.