研究了1株高富硒率乳酸菌短乳杆菌(Lactobacillus brevis)CGMCC 6683在降解Na2SeO3过程中的生长情况。结果显示，短乳杆菌在含有Na2SeO3的培养基中生长24 h后，生物量由108 CFU/mL最多下降至104 CFU/mL，菌体富硒后其膜表面形态发生变化甚至产生破损。实时荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction，qRT-PCR)结果显示，编码甲硫氨酸亚砜还原酶以及硫氧还蛋白还原酶的基因发生显著上调，表明菌体内被诱导处于氧化应激状态，后续丙二醛含量检测结果与此互为印证，表明菌体内脂质过氧化反应的发生。综合表明，菌体代谢Na2SeO3过程中体内处于氧化应激状态，产生的反应活性氧与菌体膜脂质发生脂质过氧化反应，造成膜受损，最终导致菌体死亡。

The growth of a lactobacillus named Lactobacillus brevis CGMCC 6683 in media containing inorganic salt Na2SeO3 was studied. The results showed that the bacteria biomass decreased from 108 CFU/mL to 104 CFU/mL, and the morphologies of L. brevis were damaged according to the scanning electron microscope and laser scanning confocal microscope images. Genes encoding peptide-methionine (S/R)-S-oxide reductase and thioredoxin-disulfide reductase were upregulated significantly as indicated by real-time quantitative polymerase chain reaction (qRT-PCR), it showed that oxidative stress was occurred in L.brevis while growing in the Na2SeO3 contained medium, this was in line with the malondialdehyde (MDA) content test result which accounted for the occurrence of lipid peroxidation. In conclusion, reaction between reaction active oxygen (ROS) and membrane lipids could cause membrane damages and resulted in death of cells ultimately.