为促进齐整小核菌(Sclerotium rolfsii WSH-G01)利用葡萄糖生产硬葡聚糖，在确定了最优搅拌转速的基础上，研究了2种补料方式对菌体生物量和多糖产量的影响。确定了控制搅拌转速400 r/min；发酵40 h时开始，恒速流加补料葡萄糖总浓度400 g/L，流速6 g/(L·h)，补料维持时间32 h的补料策略是最佳补料调控策略。结果表明，此种补料方式在齐整小核菌发酵72 h时获得最高硬葡聚糖产量32.62 g/L，生物量22.36 g/L，生产强度0.45 g/(L·h)，底物利用率28%。多糖产量较另一种补料方式提高了15%，较分批发酵提高了76.5%。

In order to improve the production of scleroglucan by Sclerotium rolfsii WSH-G01 using glucose as the substrate, on the basis of the optimal stirring speed, the effects of two feeding methods on biomass and titer of polysaccharides were studied. The stirring speed was 400 r/min. The best feeding strategy was maintaining the feeding speed of 6 g/(L·h) for 32 h after 40 h of fermentation when the total glucose concentration was 400 g/L. The results showed that the highest titer of polysaccharide (32.62 g/L), the biomass (22.36 g/L), the productivity (0.45 g/(L·h)), and the substrate utilization rate (28%) were reached at 72 h. The polysaccharide production was 15% higher than that obtained using the other feeding method and increased by 76.5% compared with batch fermentation.