采用型号为T-258和7302的2种聚醚改性硅为主要活性组分，以疏水白炭黑、羧甲基纤维素钠(sodium carboxymethyl cellulose，CMC)、去离子水为辅料，采用转相法，将水相分批加入油相，复配得到一种自乳化的高效发酵用消泡剂。实验表明，在乳化时间30 min，乳化温度65 ℃，T-258与7302质量比为1∶2(总质量分数30%)，增稠剂CMC质量分数为0.8%，结合质量分数为1%的疏水白炭黑的条件下，消泡剂具有良好的外观状态，整体呈均匀稳定的白色乳液，消泡迅速，抑泡时间长。在模拟氨基酸发酵液中铺展性好，消泡时间约3～5 s，抑泡95 min左右。且在126 ℃蒸汽灭菌1 h后基本保持稳定均一状态。

A kind of self-emulsifying defoaming agent using two polyether modified silicones T-258 and 7302 as the main active components, hydrophobic silica, sodium carboxymethyl cellulose (CMC), deionized water as the auxiliary materials, was obtained using phase inversion method through adding water phase into oil phase gradually. The defoaming agent could be used in fermentation efficiently and showed well appearance of uniform and stable white emulsion under the following conditions: 30 minutes of emulsifying time，65 ℃ of emulsifying temperature, the mass ratio of T-258 and 7302 was 1∶2 (total mass fraction was 30%)， the addition amount of thickening agent was 0.8%， combined with 1% of hydrophobic silica. Meanwhile, the defoaming agent could eliminate the foam quickly in 3-5 s and had a long foam-inhibiting period of 95 minutes in the simulated amino acid fermentation system due to its excellent spreadability. Also the defoaming agent could maintain a stable and uniform state after steam sterilization at 126 ℃ for 1 h.