为了阐明大肠杆菌卟啉合成途径关键酶基因和环境因素对卟啉代谢的调控作用,分别同源过表达了gltX、 hemA、 hemB、 hemD、 hemH及hemAD基因,分析了环境因素对卟啉前体5-氨基乙酰丙酸(5-aminolevulinic, ALA)和终产物血红素合成的影响。结果表明,hemA过表达显著促进了卟啉和血红素合成,温度和溶氧条件对hemA重组菌Eco/pEA产ALA与血红素的影响不一致。在最佳环境条件下,Eco/pEA的血红素含量是原始菌株的11.7倍。因此,不同关键酶基因的过表达与环境因素对卟啉代谢的影响存在显著差异。
In order to elucidate the effects of the genes encoding key enzymes in the porphyrin synthetic pathway as well as environmental factors on the regulation of porphyrin metabolism in E.coli, genes of gltX, hemA, hemB, hemD hemH and hemA-hemD were homogenously overexpressed respectively, and the effects of environmental factors on the precursor 5-aminolevulinic (ALA) and heme biosynthesis were analyzed. The results showed that the overexpression of hemA promoted the synthesis of heme obviously, and the effects of temperature and dissolved oxygen on the production of ALA and heme in Eco/pEA, a hemA overexpressed strain, were inconsistent. Under the optimal condition, the heme content in Eco/pEA was 11.7 fold of that in the parent strain. Therefore, the differences were significant among gene-overexpressing strains under different environmental conditions in porphyrin metabolism.
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