该文研究了甘露寡糖(mannan-oligosaccharide, MOS)对高脂饮食(high-fat diet, HFD)小鼠的益生作用。设置正常饮食(normal chow, NC)、正常饮食灌胃MOS (NC+MOS)、高脂饮食(HFD)及高脂饮食灌胃MOS (HFD+MOS)共4个实验组,MOS灌胃量为每天6 g/kg小鼠体重。经过11周实验处死小鼠,通过16 s高通量测序技术检测最后1周小鼠粪便中肠道微生物的组成变化,利用LEfSe找出标志微生物,利用PICRUSt对肠道微生物功能组成进行预测,并运用气相色谱检测小鼠盲肠内容物短链脂肪酸含量。结果显示,MOS能够有效减缓高脂饮食造成的小鼠体重增长;同时改变了高脂及正常饮食小鼠的肠道菌群结构,选择性地调节了一些微生物,增加了Akkermansia, Lactobacillus和Bifidobacterium等益生菌的相对丰度;PICRUSt分析结果显示,MOS能够提高小鼠肠道菌群脂代谢的能力;MOS还提高了正常及高脂饮食小鼠盲肠中乙酸、丙酸、丁酸的含量,同时降低了戊酸的含量。MOS能够促进高脂饮食小鼠减轻体重,调节肠道菌群结构,促进益生菌的增殖,增强肠道菌群脂代谢能力,并增加一些短链脂肪酸含量,对高脂饮食造成的肥胖小鼠具有良好的益生作用。
The effect of mannan-oligosaccharide (MOS) on high-fat diet (HFD) mice was studied. Four experimental groups were set as normal chow (NC), normal chow gavage with MOS (NC+MOS), high fat diet (HFD) and high fat diet gavage with MOS (HFD+MOS). The dosage of MOS was 6 g/kg body weight per day. After 11 weeks of experiment, mice were sacrificed and the gut microbiota of the mice at 11th week were detected by 16s high-throughput sequencing. LEfSe was used to identify the marker microorganisms. PICRUSt was used to predict the functional composition of gut microbiota, and gas chromatography was used to detect the concentrations of short-chain fatty acids (SCFAs) in cecal content. The results showed that MOS could significantly reduce the body weight gain of HFD mice. Supplement with MOS could not only change the structure of gut microbiota in both HFD and NC mice, but also selectively modulate some microbiome and increase the relative abundance of Akkermansia, Lactobacillus and Bifidobacterium etc. PICRUSt analysis showed that MOS enhanced lipid metabolism of gut microbiota in mice. Meanwhile, supplement with MOS increased the concentrations of acetic, propionic and butyric acid and decreased the concentration of valeric acid in cecal content of mice. The above results showed that MOS could not only help the weight-loss of HFD mice and modulate the gut microbiota structure, but also promote the proliferation of probiotics and enhance lipid metabolism function of gut microbiota with the increasing content of some SCFAs, Thus MOS had good probiotic effect on obese mice caused by high-fat diet.
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