荧光假单胞菌对采后锦橙青霉病的防治和贮藏品质的影响

  • 王智荣 ,
  • 江孟遥 ,
  • 杜木英 ,
  • 汪开拓 ,
  • ZsoltZalán ,
  • FerencHegyi ,
  • 阚建全
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  • 1(西南大学 食品科学学院,重庆,400715)
    2(中匈食品科学联合研究中心,重庆,400715)
    3(农业部农产品贮藏保鲜质量安全风险评估实验室(重庆),重庆,400715)
    4(重庆三峡学院 生命科学与工程学院,重庆,404100)
    5(匈牙利国家农业研究和创新中心食品科学研究所,匈牙利布达佩斯,H-1022)
硕士研究生(阚建全教授为通讯作者,E-mail:kanjianquan@163.com)。

收稿日期: 2018-04-29

  网络出版日期: 2018-12-25

基金资助

中国同匈牙利政府间国际科技创新合作重点专项(国家重点研发计划资助,中国编号为2016YFE0130600,匈牙利编号为TET_16_CN-1-2016-0004)

Effects of Pseudomonas fluorescens on controlling blue mold caused by Penicillium italicum in Jincheng citrus fruits and its storage quality

  • WANG Zhi-rong ,
  • JIANG Meng-yao ,
  • DU Mu-ying ,
  • WANG Kai-tuo ,
  • Zsolt Zalán ,
  • Ferenc Hegyi ,
  • KAN Jian-quan
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  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, China)
    3(Laboratory of Quality & Safety Risk Assessment for Agro-Products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing 400715, China)
    4(College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, China)
    5(Food Science Research Institute of National Agricultural Research and Innovation Center, Budapest H-1022, Hungary)

Received date: 2018-04-29

  Online published: 2018-12-25

摘要

以“北碚447”锦橙果实为实验材料,研究了荧光假单胞菌(Pseudomonas fluorescens)对采后锦橙青霉病的防治效果和贮藏品质及感官品质的影响。利用P. fluorescens不同浓度菌悬液和各种处理液分别进行离体和活体实验。离体实验结果表明,1 × 108 CFU/mL菌悬液在混合固体培养基上和意大利青霉孢子共同培养时,能完全抑制其生长繁殖,对峙培养时,相对抑菌率高达(52.59±1.59)%;在马铃薯葡萄糖液体培养基中意大利青霉孢子12 h后的萌发率和芽管长度分别为(0.67±0.47)%和(2.25±0.25)μm。活体实验结果表明,P. fluorescens菌悬液抑菌效果最好,且其浓度越高,防治效果越好,浓度为1 × 109 CFU/mL时,果实的发病率和病斑直径仅为(49.17±1.18)%和(2.72±0.09)cm;4 ℃下培养16 d,20 ℃下培养8 d,P. fluorescens在果实伤口处的活菌数分别增长了28倍和34倍;和对照相比,P. fluorescens能显著提高果实几丁质酶和β-1,3-葡聚糖酶的活性,有效增强果实的抗病性;经P. fluorescens菌悬液保鲜的果实,病情指数显著降低,且感官品质和对照差别较小,表明该P. fluorescens试验菌株在锦橙采后病害上具有极高的生物防治潜力。

本文引用格式

王智荣 , 江孟遥 , 杜木英 , 汪开拓 , ZsoltZalán , FerencHegyi , 阚建全 . 荧光假单胞菌对采后锦橙青霉病的防治和贮藏品质的影响[J]. 食品与发酵工业, 2018 , 44(11) : 176 -184 . DOI: 10.13995/j.cnki.11-1802/ts.017668

Abstract

The present study was conducted to investigate the effects of treatments with Pseudomonas fluorescens on controlling blue mold caused by Penicillium italicum and storage quality and sensory quality of postharvest Jincheng citrus fruits. In vitro and in vivo experiments were carried out by using different concentrations of P. fluorescens suspension and various processing fluids. In vitro results showed that P. fluorescens could completely inhibit the growth of P. italicum when it was incubated with spore suspension of P. italicum in agar plate. The relative inhibition rate was up to (52.59±1.59)% when it was cultured confronting against P. digitatum. The spore germination rate and germ tube length was only (0.67±0.47)% and (2.25±0.25) μm, respectively, when the concentration of bacterial suspension was 1×108 CFU/mL. In vivo results showed that the inhibitory effects of P. fluorescens suspension on blue mold on postharvest Jincheng orange was related to the concentration. The higher concentration, the disease incidence would be lower and the lesion diameter would be smaller. When the bacterial suspension was applied at 1.0×109 CFU/ml, the disease incidence and lesion diameter was only (49.17±1.18)% and (2.72±0.09) cm, respectively. Moreover, the population of P. fluorescens was increased by approximately 28- and 34-fold after incubation at 20 ℃ for 8 days and 4 ℃ for 16 days, respectively. Furthermore the disease resistance of Jincheng could be enhanced by P. fluorescens through inducing an increase of Chitinase and β-1,3-glucanase. P. fluorescens could effectively inhibit the disease of fruits, and there was little differences on sensory quality between treatment group with P. fluorescens and control group. Based on the above analysis, the P. fluorescens strain had high biological control potential in the postharvest diseases of Jincheng citrus fruits.

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