以雨生红球藻(Haematococcus pluvialis) LUGU为对象,研究了高光照联合缺氮条件下,外源添加褪黑素(melatonin, MLT)对H. pluvialis生物量、虾青素积累量、油脂含量以及与虾青素、油脂合成相关酶基因表达量的影响。结果显示:缺氮胁迫、联合高光诱导条件下,外源添加10 μmol/L MLT,虾青素积累量显著提高,最大虾青素积累量可达32.37 mg/g,是对照组的2.25倍;藻细胞内油脂的含量达到42.84%,为对照组的1.21倍。虾青素合成关键酶基因bkt及油脂合成关键酶基因fad的表达量均出现不同程度的上调。此外,研究了外源MLT对细胞内活性氧(ROS)水平的影响以及相关抗氧化酶的活性变化规律。 ROS水平受到抑制,虾青素合成相关的抗氧化酶SOD、CAT和POD活性增强。研究表明,在高光照联合缺氮胁迫条件下,外源添加MLT有利于雨生红球藻积累虾青素,为强化虾青素的生物合成提供了理论依据。
The effects of melatonin (MLT) on biomass density, accumulation of astaxanthin, lipid synthesis, and expression level of the genes associated with astaxanthin accumulation and lipid synthesis in Haematococcus pluvialis LUGU was investigated under the conditions of high light and nitrogen deficiency cultivation process was stud. The results showed that under nitrogen deficiency combined with high light and induced exogenous addition of 10 μM MLT, astaxanthin accumulation was significantly increased up to 32.37 mg/g, 2.25 folds of the control, and the intracellular lipid reached 42.84%, 1.21 folds of the control. The expression of bkt, the key gene for the accumulation of astaxanthin, and fad, the key gene for the synthesis of lipid, were also up-regulated. In addition, the effects of MLT on intracellular reactive oxygen species (ROS) levels and the activity changes of antioxidant enzymes that related to the synthesis of astaxanthin were investigated. ROS synthesis was inhibited and the activities of SOD, CAT, and POD were enhanced. That exogenous addition of MLT facilitated Haematococcus pluvialis the accumulation of astaxanthin under conditions of high light and nitrogen deficiency stress provides a theoretical basis for enhancing the biosynthesis of astaxanthin.
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