建立了食品用金属罐中苯酚、双酚A、间甲基苯酚、邻甲基苯酚、4-叔丁基苯酚迁移量的超高效液相色谱结合荧光检测器的分析方法,并开展了食品用金属罐中相关游离酚的迁移水平分析。5种游离酚在0.010~0.500 mg/L的范围内线性关系良好;在水、4%乙酸溶液、10%乙醇溶液和玉米油4种不同模拟物中加标回收率为70.2%~118.7%,平均回收率为90.7%,精密度范围为0.9%~7.2%(n=6);5种游离酚的检出限范围为0.001~0.010 mg/L。该方法准确性高、重现性好,可用于食品用金属罐中游离酚迁移量的检测分析。对6类共49种食品用金属罐采用6种不同食品模拟物浸泡,依照迁移实验条件进行游离酚迁移量分析。结果显示,除4-叔丁基苯酚之外,其他各游离酚的迁移量与总游离酚迁移量均处于限量值之内,双酚A对总游离酚的迁移量贡献最大。不同种类模拟物以及不同涂层、不同罐型条件下,5种游离酚的迁移量与检出率存在一定差异。
A analytical method of ultra-high performance liquid chromatography with fluorescence detector was established to detect migration of phenol, bisphenol A, methylphenol, o-methylphenol, and 4-tert-butylphenol from metal cans intend to contact with food, and the migration risk of related free phenols in metal cans was investigated. The 5 free phenols had a good linear relationship between 0.010 mg/L and 0.500 mg/L. In 4 different simulants of water,4% acetic acid,10% ethanol solution and corn oil, the recovery rate was in the range of 70.2%~118.7% and the average was 90.7%. The range of accuracy was 0.9%~7.2%(n=6). The detection limits ranged between 0.001~ 0.010 mg/L for 5 free phenols in 4 different simulants. The method has high accuracy and good reproducibility and can be used for analysis of free phenol migration in metal cans intend to contact with food. A total of 49 kinds of metal cans were soaked by corresponding food simulants, and the migration of free phenol was analyzed according to the migration experimental conditions. The migration risk of each free phenol and total amount of free phenol were within the safe limit except 4-tert-butylphenol, and bisphenol A was biggest contributors to the total migration amount of free phenol. Migration and detection rate of 5 kinds of free phenol vary under the conditions of different simulation, coating and can type.
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