该研究以小麦醇溶蛋白为载体,阿拉伯胶为稳定剂,制备负载根皮素的小麦醇溶蛋白-阿拉伯胶纳米颗粒,以达到稳定根皮素从而发挥其药效的目的。考察了小麦醇溶蛋白质量浓度、醇水比、pH值、盐离子浓度对纳米颗粒的影响,得出最佳的实验条件为小麦醇溶蛋白储备液的质量浓度为50 g/L,醇水体积比1∶50,醇溶蛋白和阿拉伯胶的质量比为1∶2,pH值为4~9, 盐离子浓度不大于20 mmol/L,此时纳米颗粒非常稳定;当纳米颗粒与根皮素质量比为1∶5 时,包封率达到88.9%以上,且粒径、多分散性指数(polydispersity,PDI)、电位均在稳定范围内。复合阿拉伯胶的小麦醇溶蛋白纳米颗粒的稳定性、耐盐耐酸碱性、对根皮素负载率均有显著提高。

Gliadin and arabic gum nanoparticles loaded with phloretin were prepared with gliadin as carrier and arabic gum as stabilizer, so as to stabilize phloretin and exert its efficacy. The effects of the concentration of gliadin, the ratio of the ethanol to water, pH and the concentration of salt on the stability of nanoparticles were investigated. High content tocopherol (phloretin) -AG nanoparticle was also prepared. The results showed that the best conditions were as follows: 5% of Gliadin, 1∶50 ratio of ethanol∶water, Gliadin∶AG=1∶2, at pH 4-9, less than 20 mmol /L of salt concentration. Under these conditions, the Gliadin -AG nanoparticles were very stable. When the ratio of Gliadin -AG nanoparticles to phloretin was 1∶5, the encapsulation efficiency was more than 88.9%, and the particle size, PDI, zeta-potential of the nanoparticles were all stable. The stability of compound nanoparticle of AG and Gliadin, as well as the tolerance of acid, alkali and salt all improved the encapsulation efficiency of phloretin.