废杂糖的资源化利用是高果糖浆生产行业迫切需要解决的问题。该研究首先通过高效液相、质谱和红外光谱分析,确定了杂糖成分为葡萄糖、果糖和聚合度为2~16的线性葡聚糖,包括葡萄糖480 g/L,果糖92 g/L,麦芽糖103.6 g/L,麦芽三糖36.8 g/L,总糖含量802.3 g/L。进一步使用2种常用的毕赤酵母宿主(PAOX1型毕赤酵母、PGAP型毕赤酵母)利用杂糖发酵生产内切β-1,3葡聚糖酶并与标准碳源(甘油和葡萄糖)作对比。结果表明,对于PAOX1型毕赤酵母,杂糖做碳源时细胞密度和酶活性与甘油相比均有所下降,最大生物量分别为59.1和82.0 g/L,最高酶活性分别为157.29和199.2 U/mL。对于PGAP 型毕赤酵母,杂糖与葡萄糖的发酵效果相当,说明杂糖可以作为PGAP型毕赤酵母生产内切β-1,3葡聚糖酶的优质替代性碳源。
The production of high-fructose syrup generates great quantities of waste liquor, which contains miscellaneous sugar. Efficient utilization of this mixed carbohydrate is an urgent problem that needs to be solved. In this study, we first determined the components of the mixed carbohydrate, which were glucose, fructose, and the linear glucans with a degree of polymerization ranging from 2-16, using HPLC, MALDI-MS, and FTIR. Specifically, it contained glucose 480 g/L, fructose 92 g/L, maltose 103.6 g/L and maltotriose 36.8 g/L. And the total sugar was 802.3 g/L. Compared with the standard carbon sources (glycerol and glucose), this mixed carbohydrate was used in the production of endo-β-1,3-glucanase by two common types of Pichia pastoris (Pichia pastoris PAOX1 and PGAP). The results showed that, for Pichia pastoris PAOX1, when the mixed carbohydrate was used as carbon source, cell density was lower than the standard carbon sources. And the maximum biomass (DCW) was 82.0 and 59.1 g/L, respectively. Meanwhile, the enzyme activity was also lower than control samples which reached 199.2 and 157.29 U/mL respectively. However, for Pichia pastoris PGAP, the fermentation effect of the mixed carbohydrate and glucose was roughly the same, which indicated that the mixed carbohydrate can be used a s a high-quality alternative carbon source for Pichia pastoris PGAP to produce endo-β-1,3-glucanase.
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