研究报告

陕西茯砖茶的微生物多样性和群落结构

  • 杨吉霞 ,
  • 曾祥平 ,
  • 蒲慧敏 ,
  • 杨夏禾 ,
  • 贺稚非
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  • 1 (西南大学 食品科学学院,重庆,400715)
    2 (重庆市计量质量检测研究院第一分院,重庆,402260)
博士,副教授(贺稚非教授为通讯作者,E-mail:2628576386@qq.com)。

收稿日期: 2019-08-22

  网络出版日期: 2020-03-13

基金资助

重庆市基础科学与前沿技术研究项目(cstc2017jcyjAX0249);西南大学中央高校基本科研业务费项目(XDJK2018C070,XDJK2015C135,SWU113035),四川省教育厅川菜发展研究中心科研项目(CC18Z20)

Investigation of the microbial diversity and community structure in Shaanxi Fu brick tea

  • YANG Jixia ,
  • ZENG Xiangping ,
  • PU Huimin ,
  • YANG Xiahe ,
  • HE Zhifei
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  • 1 (College of Food Science, Southwest University, Chongqing 400715, China)
    2 (Chongqing Academy of Metrology and Quality Inspection of First Branch, Chongqing 402260, China)

Received date: 2019-08-22

  Online published: 2020-03-13

摘要

陕西是茯砖茶的起源地和主产区之一,为分析陕西茯砖茶微生物组成,采用Illumina MiSeq对6个样品中细菌16S rRNA基因和真菌ITS1基因测序并做序列分析。结果显示,只有sx4和sx5两个样品检出细菌,优势属包括Enterococcus、Lactobacillus、Akkermansia、Parabacteroides、Faecalibacterium。真菌的优势属为Aspergillus,平均相对丰度为99.123%,最优势的种可能为Aspergillus amstelodami、Aspergillus chevalieri、Eurotium sp.、Aspergillus spiculosus、Aspergillus heterocaryoticus、Aspergillus sp.、Aspergillus ruber、Aspergillus cristatus中的一种或几种,还包括曲霉Aspergillus cibarius(4.138%)、Aspergillus piperis(0.879%)、Aspergillus penicillioides(0.019%)、Aspergillus gracilis(0.0058%);酶母Candida、Cyberlindnera、Cryptococcus、Guehomyces;霉菌Mortierella、Humicola、Fusarium、Gibberella、Phoma。该研究阐述了陕西茯砖茶样品中细菌和真菌的多样性和群落结构,可为地理标志产品保护提供参考。

本文引用格式

杨吉霞 , 曾祥平 , 蒲慧敏 , 杨夏禾 , 贺稚非 . 陕西茯砖茶的微生物多样性和群落结构[J]. 食品与发酵工业, 2020 , 46(3) : 50 -57 . DOI: 10.13995/j.cnki.11-1802/ts.022055

Abstract

Fu brick tea is a kind of post-fermented tea, and microorganisms play a critical role in the formation of its unique qualities. Shaanxi is the “birthplace” of Fu brick tea, and also one of the major producing areas. This study aimed to analyze the microbial profiles of Shaanxi Fu brick tea. Six Fu brick tea samples were collected from Shaanxi province, their 16S rRNA genes and ITS1 genes were sequenced with the use of Illumina MiSeq technology, and then were analyzed with Quantitative Insights Into Microbial Ecology (QIIME) pipeline. The results showed that only sx4 and sx5 contained bacteria and the dominant genera including Enterococcus, Lactobacillus, Akkermansia, Paracharacteroids and Faecalibacterium. The dominant fungi was Aspergillus, with an average relative abundance of 99.123%. At species level, one or more following species may involve in Fu brick tea samples which including Aspergillus amstelodami, Aspergillus chevalieri, Eurotium sp., Aspergillus spiculosus, Aspergillus hetrocaryoticus, Aspergillus sp., Aspergillus ruber and Aspergillus cristatus. Besides, species with lower abundance such as Aspergillus cibarius (4.138%), Aspergillus piperis (0.879%), Aspergillus penicillioides (0.019%) and Aspergillus gracilis (0.0058%) Candida, Cyberlindnera, Cryptococcus, Guehomyces, Mortierella, Humicola, Fusarium, Gibberella, Phoma were also be found in the collected samples. This study provided the basic diversity and community structure of microorganisms in Shaanxi Fu brick tea, and may help for the protection of Fu brick tea as the geographical indication products.

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