陕西是茯砖茶的起源地和主产区之一,为分析陕西茯砖茶微生物组成,采用Illumina MiSeq对6个样品中细菌16S rRNA基因和真菌ITS1基因测序并做序列分析。结果显示,只有sx4和sx5两个样品检出细菌,优势属包括Enterococcus、Lactobacillus、Akkermansia、Parabacteroides、Faecalibacterium。真菌的优势属为Aspergillus,平均相对丰度为99.123%,最优势的种可能为Aspergillus amstelodami、Aspergillus chevalieri、Eurotium sp.、Aspergillus spiculosus、Aspergillus heterocaryoticus、Aspergillus sp.、Aspergillus ruber、Aspergillus cristatus中的一种或几种,还包括曲霉Aspergillus cibarius(4.138%)、Aspergillus piperis(0.879%)、Aspergillus penicillioides(0.019%)、Aspergillus gracilis(0.0058%);酶母Candida、Cyberlindnera、Cryptococcus、Guehomyces;霉菌Mortierella、Humicola、Fusarium、Gibberella、Phoma。该研究阐述了陕西茯砖茶样品中细菌和真菌的多样性和群落结构,可为地理标志产品保护提供参考。
Fu brick tea is a kind of post-fermented tea, and microorganisms play a critical role in the formation of its unique qualities. Shaanxi is the “birthplace” of Fu brick tea, and also one of the major producing areas. This study aimed to analyze the microbial profiles of Shaanxi Fu brick tea. Six Fu brick tea samples were collected from Shaanxi province, their 16S rRNA genes and ITS1 genes were sequenced with the use of Illumina MiSeq technology, and then were analyzed with Quantitative Insights Into Microbial Ecology (QIIME) pipeline. The results showed that only sx4 and sx5 contained bacteria and the dominant genera including Enterococcus, Lactobacillus, Akkermansia, Paracharacteroids and Faecalibacterium. The dominant fungi was Aspergillus, with an average relative abundance of 99.123%. At species level, one or more following species may involve in Fu brick tea samples which including Aspergillus amstelodami, Aspergillus chevalieri, Eurotium sp., Aspergillus spiculosus, Aspergillus hetrocaryoticus, Aspergillus sp., Aspergillus ruber and Aspergillus cristatus. Besides, species with lower abundance such as Aspergillus cibarius (4.138%), Aspergillus piperis (0.879%), Aspergillus penicillioides (0.019%) and Aspergillus gracilis (0.0058%) Candida, Cyberlindnera, Cryptococcus, Guehomyces, Mortierella, Humicola, Fusarium, Gibberella, Phoma were also be found in the collected samples. This study provided the basic diversity and community structure of microorganisms in Shaanxi Fu brick tea, and may help for the protection of Fu brick tea as the geographical indication products.
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