探讨日粮添加表没食子儿茶素没食子酸酯(epigallo-catechin gallate,EGCG)对鸡肉在冷藏过程中品质和抗氧化性的影响,以揭示EGCG对鸡肉的非酶抗氧化机理。向肉鸡日粮添加EGCG 100 mg/kg(以100%纯度计)、200 mg/kg维生素E,饲喂21 d后宰杀,以鸡胸肉为研究对象,在4 ℃贮藏21 d,测定鸡肉在冷藏过程中氧化稳定性和品质指标,以及鸡肉中非酶抗氧化物质的含量。结果表明,随着冷藏时间延长,氧化油脂组、氧化油脂+EGCG组、VE组、EGCG组和空白组的硫代巴比妥酸(thiobarbituric acid reactive substance, TBARS)和羰基值均显著增加(P<0.05),与氧化油脂组和空白组相比较,EGCG显著抑制了TBARS和羰基值的增加,并降低了鸡肉在冷藏过程中的保水性,抑制了高铁肌红蛋白的形成速度(P<0.05)。添加EGCG能明显地提高机体中还原型谷胱甘肽(glutathione, GSH)、VE的含量及抗氧化能力,提高鸡肉在加工及贮藏过程中的氧化稳定性。
[1] 刘超,陈若芸.儿茶素及其类似物的化学和生物活性研究进展[J].中国中药杂志, 2004,10: 1 017-1 021.
[2] LI X, SUN C, LUO L, et al. Determination of tea polyphenols content by infrared spectroscopy coupled with iPLS and random frog techniques[J]. Computers and Electronics in Agriculture, 2015,112: 28-35.
[3] MARCHESE A, COPPO E, SOBOLEV A P, et al. Influence of in vitro simulated gastroduodenal digestion on the antibacterial activity, metabolic profiling and polyphenols content of green tea (Camellia sinensis) [J]. Food Research International, 2014, 63: 182-191.
[4] GOBERT M, RÉMOND D, LOONIS M, et al. Fruits, vegetables and their polyphenols protectdietary lipids from oxidation during gastric digestion[J]. Food Function, 2014, 5: 2 166-2 174.
[5] MAŁGORZATA K, ZBIGNIEW J D. Effect of mustard on lipid oxidation in model pork meat product[J]. European Journal of Lipid Science and Technology, 2014, 116(3): 311-318.
[6] 贾娜,刘丹,王乐田,等. 儿茶素对冷藏猪肉糜脂肪和蛋白氧化及品质特性的影响[J]. 中国食品学报,2018, 18(12):210-218.
[7] PREDESCU C, PAPUC C, PETCU C, et al. The effect of some polyphenols on minced pork during refrigeration compared with ascorbic acid[J]. Bulletin UASVM Food Science and Technology, 2018, 75(1): 36-42.
[8] 李卫春, 焦卫民,刘福柱,等. 日粮添加茶多酚对肉鸡生产性能及肌肉抗氧化性能的影响[J].西北农林科技大学学报(自然科学版), 2008, 11: 16-20;28.
[9] 崔帅, 王光,郑菲菲,等. 饲粮中添加茶多酚对育成前期水貂生长性能及营养物质消化率的影响[J].中国畜牧杂志, 2017, 53(9): 121-125.
[10] 王红芹. 茶多酚和维生素E对肉仔鸡抗氧化性能的影响[J]. 安徽农业科学, 2012, 40(8): 4 588-4 589;4 594.
[11] 李永义, 段绪东,赵娇,等. 茶多酚对氧化应激仔猪生长性能和免疫功能的影响[J]. 中国畜牧杂志, 2011, 47(15): 53-57.
[12] ZHONG R Z, TAN C Y, HAN X F, et al. Effect of dietary tea catechins supplementation in goats on the quality of meat kept under refrigeration[J]. Small Ruminant Research, 2009, 87(1-3):122-125.
[13] 晁娅梅,陈代文,余冰,等,茶多酚对育肥猪生长性能、抗氧化能力、胴体品质和肉品质的影响[J]. 动物营养学报, 2016, 28(12): 3 996-4 005.
[14] SAHIN K, ORHAN C, TUZCU M, et al. Epigallocatechin-3-gallate prevents lipid peroxidation and enhances antioxidant defense system via modulating hepatic nuclear transcription factors in heat-stressed quails[J]. Poultry Science, 2010, 89(10):2 251-2 258.
[15] ZHANG Y, LUO H, LIU K, et al. Antioxidant effects of liquorice (Glycyrrhiza uralensis) extract during aging of longissimus thoracis muscle in Tan sheep[J]. Meat Science, 2015, 105: 38-45.
[16] ROSSI R, PASTORELLI G, CANNATA S, et al. Effect of long term dietary supplementation with plant extract on carcass characteristics meat quality and oxidative stability in pork[J]. Meat Science, 2013, 95(3): 542-548.
[17] ZHONG R, FANG Y, QIN G X, et al. Tea catechins protect goat skeletal muscle against H2O2-induced oxidative stress by modulating expression of phase 2 antioxidant enzymes[J]. Journal of Agricultural and Food Chemistry, 2015, 63(36): 7 921-7 928.
[18] SINNHUBER R O, YU T. The 2-thiobarbituric acid reaction, an objective measure of the oxidative dete rioration occurring in fats and oils[J]. Journal of Japan Oil Chemists' Society, 1977, 25: 259-267.
[19] XIA X, KONG B, LIU Q, et al. Physicochemical change-and protein oxidation in porcine longissimus dorsi as influenced by different freeze-thaw cycles[J]. Meat Science, 2009, 83(2): 239-245.
[20] LIU Z, XIONG YL, CHEN J. Protein oxidation enhances hydration but suppresses water-holding capacity in porcine longissimus muscle[J]. Journal of Agricultural and Food Chemistry, 2010, 58(19): 10 697-10 704.
[21] MOŇINO I, MARTíNEZ C, SOTOMAYOR JA, et al. Polyphenolic transmission to segureno lamb meat from ewes’ diet supplemented with the distillate from rosemary (Rosmarinus offcinalis) leaves[J]. Agricultural and Food Chemistry, 2008, 56(9): 3 363-3 367.
[22] 佀凤为,黄莉,孔琳. 加工工序对香椿芽抗氧化性影响的研究[J].食品研究与开发, 2017, 38(6): 6-10.
[23] TANG S Z, KERRY J P, SHEEHAN D, et al. Antioxidative mechanisms of tea catechins in chicken meat systems[J]. Food Chemistry, 2002, 76(1): 45-51.
[24] ALGHAZEER R, SAEED S, HOWELL N K. Aldehyde formation in frozen mackerel (Scomber scombrus) in the presence and absence of instant green tea[J].Food Chemistry, 2008, 108(3): 801-810.
[25] MORZEL M, GATELLIER P, SAYD T, et al. Chemical oxidation decreases proteolytic susceptibility of skeletal muscle myofibrillar proteins[J]. Meat Science, 2006, 73(3): 536-543.
[26] LUND M N, HEINONEN M, BARON C P, et al. Protein oxidation in muscle foods: A review[J]. Molecular Nutrition & Food Research, 2011, 55(1): 83-95.
[27] 王汉玲,刘彩华,秦军委,等. 羟自由基氧化系统对草鱼肌肉蛋白理化特性及其保水性的影响[J]. 食品科技, 2018, 43(1): 134-140.
[28] O'GRADY N M, MONAHAN F J, BRUNTON N P. Oxymyoglobin oxidation and lipid oxidation in bovine muscle-mechanistic studies[J]. Journal of Food Science, 2001, 66(3): 387-392.
[29] 臧鹏,屈维丽,于燕波,等. 表没食子儿茶素没食子酸酯在食品体系中的抗氧化作用研究[J]. 食品工业科技,2011,32(11):361-363.
[30] KARAMI M, ALIMON A R, GOH Y M. Effect of vitamin E, Andrographis paniculata and turmeric as dietary antioxidant supplementation on lipid and color stability of goat meat[J]. Small Ruminant Research, 2011, 97(1-3): 67-71.
[31] 刘旭升,黄进宝,宛晓春,等. 饲料中添加绿茶粉对鸡肉肌苷酸和维生素E含量的影响[J]. 食品工业科技,2015,36(19):370-373;378.
[32] 唐雪莲,李志华,付京花. 维生素E对吉富罗非鱼肝脏谷胱甘肽含量及其相关酶活力的影响[J]. 黑龙江畜牧兽医,2013(15):10-14.
[33] ORTUŇO J, SERRANO R, BAŇÓN S. Antioxidant and antimicrobial effects of dietary supplementation with rosemary diterpenes (carnosic acid and carnosol) vs vitamin E on lamb meat packed under protective atmosphere[J]. Meat Science, 2015, 110: 62-69.