该文探讨一种含L-精氨酸和抗氧化物质(山楂提取物、知母提取物和虾青素)组方对小鼠免疫功能的影响。将SPF级雄性ICR小鼠随机分为四组,分别为对照组和受试物低、中和高剂量组(0.133、0.267和0.800 g/kg·BW)。连续灌胃30 d后,测定小鼠主要免疫器官脏器系数、细胞免疫功能指标、体液免疫功能指标、非特异性免疫功能指标。结果显示,该组方(中、高剂量)能显著提高淋巴细胞增殖能力和NK细胞活性,低、中、高剂量均能显著提高小鼠巨噬细胞吞噬鸡红细胞百分率及吞噬指数。在该实验条件下,组方具有增强小鼠免疫的功能。
In this paper, the effects of a formula composed of L-argarginine, crataegus extract, chinonin extract and astaxanthin on the immune function were studied using mouse model. SPF male ICR mice were randomly divided into four groups:a control group and low, medium and high dose groups. After 30 days of continuous intragastric administration, mice’s organ coefficients, cellular immune function indexes, humoral immune function indexes and non-specific immune function indexes were measured. The results showed that medium and high doses groups of the formula could significantly increase the lymphocyte proliferation ability and NK cell activity and low, medium and high doses groups could significantly increase the percentages of phagocytic chicken erythrocytes and phagocytic index of mouse macrophages. Under the experimental conditions, this formula has the function of enhancing the immunity of mice.
[1] GEIGER R, RIECKMANN J C, WOLF T, et al. L-Arginine modulates T cell metabolism and enhances survival and anti-tumor activity[J]. Cell, 2016, 167(3):829-842.
[2] XIONG L,TENG J L,BOTELHO M G,et al. Arginine metabolism in bacterial pathogenesis and cancer therapy[J].Int J Mol Sci,2016,17(3):363.
[3] 欧阳暂,姜雅慧,张剑波等.精氨酸强化的免疫营养支持对胃癌患者术后免疫功能影响的Meta分析[J].循证医学,2017,17(6):350-360.
[4] SHEN J G, LI M, XIN W J, et al. Effects of Chinonin on nitric oxide free radical, myocardial damage and arrhythmia in ischemia-reperfusion injury in vivo[J]. Appl Magn Reson,2000,19(1):9-19.
[5] ZHANG D L, ZHANG Y T, YIN J J,et al. Oral administration of Crataegus flavonoids protects against ischemia/reperfusion brain damage in gerbils[J]. J Neur Chem,2004,90(1):211-219.
[6] 杨波,迟小华,卢学春,等.免疫细胞衰老的研究进展[J].中国实验学杂志,2012,20(3):782-787.
[7] SHEN J G, WANG J, ZHAO B L, et al. Effects of EGb-761 on nitric oxide, oxygen free radicals, myocardial damage and arrhythmias in ischemia-reperfusion injury in vivo[J]. Biochim Biophys Acta,1998,1 406(3):228-236.
[8] ZHAO B L, SHEN J G, LI M, et al. Scavenging effect of Chinonin on NO and oxygen free radicals generated from ischemia reperfusion myocadium[J]. Biachem Biophys Acta, 1996,1315(2):131-137.
[9] SHEN J G, GUO X S, JIANG B, et al. Chinonin, a novel drug against cardiomyocyte apoptosis induced by hypoxia and reoxygenation[J]. Biochim Biophyscs Acta,2000,1500(2):217-226.
[10] 赵亚硕,李砚超,杜立波,等. L-精氨酸和天然抗氧化剂对缺血性脑卒中防治作用的细胞和动物模型研究[J]. 心脑血管病防治,2016,16(4):259-263。
[11] 冯润,王红云,赵保路,等,基于一氧化氮和天然抗氧化剂的新配方的心肌保护作用[J]。中国科学院大学学报,2016,33(5):625-631.
[12] ZHAO Y, ZHAO B. Natural antioxidants in prevention and management of Alzheimer’s disease[J]. Frontiers in Bioscience, 2012,4: 794-808.
[13] 中华人民共和国卫生部.保健食品检验与评价技术规范 (2003年版)[M].北京:中华人民共和国卫生部,2003.
[14] PALMER R M, FERRIGE A G, MONCADA S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor[J]. Nature, 1987, 327(6 122): 524-526.
[15] FURCHGOTT R F, VANHOUTTE P M. Endothelium-derived relaxing and contracting factors[J]. FASEB J, 1989. 3(9): 2007-2018.
[16] 赵保路,一氧化氮自由基[M].2008,北京:科学出版社.
[17] 赵保路,一氧化氮自由基生物学和医学[M].2016,北京:科学出版社,北京.
[18] SHANTHI S, PARASAKTHY K, DEEPALAKSHMI P D, et al. Hypolipidemic activity of tincture of crataegus in rats[J]. Indian Journal of Biochemistry & Biophysics, 1994, 31(2): 143-146.
[19] WEIHMAYR T, ERNST E. Therapeutic effectiveness of Crataegus[J]. For-schritte der Medizin, 1996, 114: 27-29.
[20] LIN T, LU C, WANG S. Astaxanthin inhibits glutamate release in rat cerebral cortex nerve terminals via suppression of voltage-dependent Ca2+ entry and mitogen-activated protein kinase signaling pathway[J]. J Agric Food Chem, 2010, 58(14):8 271-8 278.
[21] NIEDBALA W,WEI XQ,PIEDRAFITA D,et al. Effects of nitric oxide on the induction and differentiation of Th1 cells[J]. Eur J Immunol,1999,29(8):2 498-2 505.
[22] HIGUERA-CIAPARA I, FELIX-VALENZUELA L, GOYCOOLEA F. Astaxanthin: a review of its chemistry and applications. Crit. Rev[J]. Food Sci Nutr 2006,46(2):185-196.
[23] WALKER A F, MARAKIS G, MORRIS A P,et al. Promising hypotensive effect of hawthorn extract: A randomized double-blind pilot study of mild, essential hypertension[J]. Phytotherapy Research: Ptr, 2002, 16(1): 48-54.
[24] PETKOV E, NIKOLOV N, UZUNOV P. Inhibitory effect of some flavonoids and falvonoid mixtures on cyclic amp phosphodiesterase activity of rat heart[J]. Planta Med, 1981, 43(2): 183-186.
[25] COLEMAN J W. Nitric oxide in immunity and inflammation[J]. Intern Immunopharm,2001,1(8):1 397-1 406.
[26] MALVEZI A D,CECCHINI R,SOUZA F,et al. Involvement of nitric oxide(NO)and TNF-α in the oxidativestress associated with anemia in experimental Trypanosoma cruzi infection[J]. FEMS Immun Med Microbiol,2004,41:69-77.
[27] VAN DER VEEN R C. Nitric oxide and T helper cell immunity[J]. Intern Immunopharm,2001,1(8):1 491-1 500.
[28] BIDRI M,FEGER F,VARADARADJALOU S,et al. Mast cells as a source and target for nitric oxide[J]. Int Immunopharmacol,2001,1(8):1 543-1 558.
[29] MIRANDA M S,SATO S,MANCINI-FILHO J. Antioxidant activity of the microalga Chlorella vulgaris cultured on special conditions[J]. Boll Chim Farm,2001,140:165-168.
