食品与发酵工业 >

2020 , Vol. 46 >Issue 8: 85 - 91

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023345

研究报告

“美早”甜樱桃褐腐病病原菌的分离及内生拮抗细菌的筛选鉴定

  • 郗良卿 ,
  • 张士凯 ,
  • 陈雨诗 ,
  • 周一宁 ,
  • 张启月 ,
  • 辛力 ,
  • 张倩 ,
  • 吴澎
展开
  • 1 (山东农业大学,山东 泰安,271000)
    2 (山东省果树研究所,山东 泰安,271000)
硕士研究生(张倩副研究员和吴澎副教授为共同通讯作者,E-mail:cherryzhang2006@126.com,13954847828@163.com)

收稿日期: 2020-01-29

  网络出版日期: 2020-05-20

基金资助

国家重点研发计划项目(2017YFD0401303);山东省果树研究所青年基金(2018KY08);山东省现代农业产业技术体系果品创新团队(SDAIT-06-13);泰安市科技发展计划(49557040-x);山东农业大学作物生物学国家重点实验室开放课题基金(2015KF14);山东省科技计划项目(J12LF03);山东省重点研发计划(2017GNC10101)

收起

Isolation of ‘Mei Zao’ sweet cherry brown rot pathogens and identification of endogenous antagonistic bacteria

  • XI Liangqing ,
  • ZHANG Shikai ,
  • CHEN Yushi ,
  • ZHOU Yining ,
  • ZHANG Qiyue ,
  • XIN Li ,
  • ZHANG Qian ,
  • WU Peng
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  • 1 (Shandong Agricultural University, Tai'an 271000, China)
    2 (Shandong Institute of Pomology, Tai'an 271000, China)

Received date: 2020-01-29

  Online published: 2020-05-20

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摘要

为明确山东泰安地区“美早”甜樱桃褐腐病的主要病原菌,并进行内生拮抗细菌的筛选鉴定,采集当地具有褐腐病特征的“美早”甜樱桃进行病原菌分离,利用形态学和分子生物学技术鉴定,并将分离的病原菌菌株回接甜樱桃进行致病性验证;针对褐腐病原菌,从健康甜樱桃本身筛选优质拮抗菌株,开展生防研究。结果显示,导致“美早”甜樱桃褐腐病的病原菌为美澳型核果褐腐病菌(Monilinia fructicola),回接试验表明,与腐败“美早”甜樱桃自然发病特征一致;从健康甜樱桃本身筛选获得内生拮抗细菌X-16,经形态学、生理生化特征以及16S rDNA序列鉴定为特基拉芽孢杆菌(Bacillus tequilensis),抑制率达到(72.27±0.55)%,对拮抗菌X-16的菌悬液、发酵液和发酵上清液进行拮抗效果验证,发酵液和发酵上清液对褐腐病菌具有较好抑制效果,抑制率分别为(71.93±0.18)%和(67.14±0.43)%。结果表明,美澳型核果褐腐病菌为山东泰安地区“美早”甜樱桃褐腐病主要病原菌;该研究筛选出的内生拮抗细菌X-16抑菌效果显著,具有优质生防菌株的潜力。

关键词： 甜樱桃; 褐腐病; 美澳型核果褐腐病菌; 内生拮抗细菌; 特基拉芽孢杆菌

本文引用格式

郗良卿 , 张士凯 , 陈雨诗 , 周一宁 , 张启月 , 辛力 , 张倩 , 吴澎 . “美早”甜樱桃褐腐病病原菌的分离及内生拮抗细菌的筛选鉴定[J]. 食品与发酵工业, 2020 , 46(8) : 85 -91 . DOI: 10.13995/j.cnki.11-1802/ts.023345

Abstract

To identify the main pathogens of sweet cherry brown rot in Tai’an, Shandong province, and to identify endogenous antagonistic bacteria, locally collected ‘Mei Zao’ sweet cherries with brown rot characteristics were used to isolate pathogens. The isolated pathogens were identified by morphology and molecular biology techniques, and the isolated pathogenic strains were returned to ‘Mei Zao’ sweet cherries for pathogenicity verification. Aiming at isolating brown rot pathogens, high-quality antagonistic strains were screened from healthy sweet cherries, and biocontrol studies were carried out. The results showed that the pathogen that caused the brown rot of ‘Mei Zao’ sweet cherry was Monilinia fructicola. The back-up test showed that it was consistent with the natural disease characteristics of the spoiled ‘Mei Zao’ sweet cherry. The endophytic antagonist X-16 was screened from healthy sweet cherry. It was identified as Bacillus tequilensis by morphology, physiological and biochemical characteristics and 16S rDNA sequence. The inhibition rate reached (72.27±0.55)%. The fermentation solution, fermentation broth and fermentation supernatant were used to verify the antagonism effect. The fermentation broth and fermentation supernatant had good inhibitory effects on brown rot pathogens, and the inhibition rates were (71.93±0.18)% and (67.14±0.43)%, respectively. Monilinia fructicola is the main pathogen of sweet cherry brown rot of ‘Mei Zao’ in Tai’an, Shandong province. The endogenous antagonistic bacteria X-16 screened in this study has significant antibacterial effect and has the potential of being a high-quality biocontrol strain.

Key words： sweet cherry; brown rot; Monilinia fructicola; endogenous antagonistic bacteria; Bacillus tequilensis

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