为了解暗褐网柄牛肝菌多糖结构,该研究以贵州兴义产野生子实体为材料,热水浸提粗多糖,经脱蛋白、脱色和柱层析纯化后,得到精制多糖AHP,用多种色谱手段表征其多糖结构,结果表明,红外光谱和核磁共振分析AHP属α型吡喃己糖,具有明显的多糖吸收峰;1-苯基-3-甲基-5-吡唑啉酮(1-phenyl-3-methyl-5-pyrazolone,PMP)柱前衍生高效液相色谱分析单糖组成为甘露糖、鼠李糖、葡萄糖、半乳糖和岩藻糖,单糖摩尔比组成为1.54∶0.07∶0.25∶1.72∶0.96;高效凝胶渗透色谱分析其分子质量为2.03×104 Da;甲基化衍生物分析表明,AHP主要对应连接方式有→1,6)-半乳聚糖,→1,3)-葡聚糖,→2,4)-甘露聚糖和→1)-半乳聚糖;电镜扫描显示多糖AHP以交错网格状为主,伴有少量球状及丝状分布。该研究表征了暗褐网柄牛肝菌多糖的结构,为进一步了解暗褐网柄牛肝菌多糖的活性机制奠定了理论基础。
In order to explorer the structure of polysaccharides from Phlebopus portentosus which was collected from Xingyi, Guizhou. The crude polysaccharide was extracted by hot water extraction method, and then successively purified by deproteinization, decoloration and column chromatography, a refined polysaccharide was obtained after purification, named AHP. The structural characteristics of AHP were elucidated by chromatographic methods. Infrared spectrometer(IR) and nuclear magnetic resonance(NMR)indicated that AHP is typical α-pyranose with characteristic peaks of polysaccharides. The monosaccharide composition was evaluated by high performance liquid chromatography(HPLC)using pre-column derivatization with 3-methyl-1-phenyl-5-pyrazolone (PMP),and AHP was found to consist of mannose, rhamnose, glucose, galactose and fucose, with a molar ratio of 1.54∶0.07∶0.25∶1.72∶0.96. The molecular weight was determined to be 2.03×104 Da by high performance gel-permeation chromatography(HPGPC).The methylated derivatives analysis suggested that the main linkage form in AHP were →1,6)-Galp,→1,3)- Glcp,→2,4)- Manp and →1)- Galp. The scanning electron microscope was used to observe AHP and showed reticular structure dominated, with a small amount of spherical and filamentous polysaccharides. This paper presents the primary structure of polysaccharide from Phlebopus portentosus. It will lay a foundation for further understanding the polysaccharide activity mechanism of Phlebopus portentosus.
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