为探究浒苔多糖(Enteromorpha prolifera polysaccharides,EPP)的结构与抗氧化活性,该研究以浙江象山浒苔为原料,采用正交实验对浒苔多糖提取工艺进行优化,用DEAE-52纤维素柱对浒苔多糖进行分离纯化,并用傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR) 对多糖结构进行表征。结果表明,浒苔多糖在粉碎目数30~40目、浸提时间90 min、浸提温度60 ℃和液料比为30∶1条件下达到最佳提取效果,提取率为16.34%;浒苔粗多糖分离纯化得到3个组分EPP-1、EPP-2、EPP-3,用凝胶渗透色谱法(gel permeation chromatography,GPC)测得其分子质量分别为121、164、162 ku;通过红外分析可知,EPP-1、EPP-2和EPP-3均为吡喃多糖,EPP-2可能是含有β-D-吡喃葡萄糖和蛋白质复合的酸性多糖,ESP-3可能为含有β-D-吡喃葡萄糖的酸性多糖。该研究结果可为浒苔的综合利用及其在医药和功能性食品行业的应用提供一定的基础依据。
In order to explore the structure and antioxidant activity of Enteromorpha prolifera polysaccharides (EPP), the extraction process of Enteromorpha prolifera polysaccharides was optimized by orthogonal experiment using Zhejiang Xiangshan Enteromorpha prolifera as raw material. The polysaccharide was separated and purified by DEAE-52 cellulose column, and its structure was characterized by Fourier transform infrared spectroscopy (FTIR). The results show that Enteromorpha prolifera polysaccharides has the best extraction effect under the conditions of 30~40 mesh crushing, 90 min extraction time, 60 ℃ extraction temperature and 30∶1 ratio of liquid to solid, and the extraction rate is 16.34%. The crude polysaccharide of Enteromorpha prolifera is separated and purified to obtain three components EPP-1, EPP-2 and EPP-3, and their molecular weights are measured by gel permeation chromatography (GPC) to be 121, 164 and 162 ku, respectively. Based on infrared analysis, EPP-1, EPP-2 and EPP-3 are pyran polysaccharide. EPP-2 may be acidic polysaccharide containing with β-D- glucopyran and protein complex. EPP-3 may be acidic polysaccharide containing with β-D- glucopyran. The results of this study can provide some basic basis for the comprehensive utilization of Enteromorpha prolifera as well as its application in medicine and functional food industry.
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