该文研究了桑叶魔芋复配粉(以下简称“复配粉”)对老年鼠高牛肉蛋白饮食全肠道及蛋白消化酶活性的影响,以期为复配粉改善老年鼠高牛肉蛋白膳食消化性提供参考。以15月龄BALB/c小鼠为受试动物,AIN-93M饲料组为阴性对照组,以牛肉蛋白含量达50%的AIN-93M饲料为试验组日粮, 其中灌胃江中牌健胃消食片为阳性对照组,灌胃不同剂量(高、中、低)复配粉为试验组,采用全肠道组织测定技术,探究复配粉对老年鼠小肠(十二指肠、空肠、回肠)、大肠(盲肠、直肠、结肠)全肠道组织结构的影响,并通过酶联免疫吸附法测定肠道中蛋白质消化关键酶(胰蛋白酶、肠肽酶)的活性。结果表明,高牛肉蛋白饮食对老年鼠小肠、大肠组织形态结构产生不利影响,而复配粉和消食片均能改善老年鼠高牛肉蛋白饮食对小鼠肠道造成的伤害。老年鼠对高蛋白饮食产生应激反应,刺激蛋白质消化酶活性显著增高,而复配粉则平缓应激反应,显著性降低蛋白酶活性,并呈现剂量关系。因此,该复配粉能改善高牛肉蛋白饮食对老年鼠肠道的损害,促进肠道健康;能够降低机体高蛋白饮食蛋白质消化酶的活性,减轻机体负担,其降低肠道蛋白酶活性而不降低蛋白质消化性的机理有待进一步研究。
In order to provide reference for improving the dietary digestibility of high beef protein in old mice, the effects of the mixture of mulberry leaf powder with konjac flour (hereinafter referred to as "compound powder") on the whole intestine structures and proteinase activities were investigated. The 15-month-old BALB/c mice were used as the experimental animals, AIN-93M standard feed as diet for negative control group (NC, gavaged with normal saline), AIN-93M with beef protein content of 50% for blank control group (BC, gavaged with normal saline) and other groups. And Jianwei Xiaoshi tablet (Jiangzhong brand) was ground and gavaged as the positive control group (PC). Applying different dosage of compound powder gavage (high dose group (HD), middle dose group (MD), and low dose group (LD)), the whole intestinal tissue measurement technology was used to explore the effect of compound powder on small intestine (duodenum, jejunum, ileum) and large intestine (cecum, rectum, colon) of old mice. The enzyme-linked immunosorbent assay (ELISA) was used to determine the activity of key enzymes (trypsin, enteropeptidase) in intestinal protein digestion. The results showed that high beef protein diet was harmful to the structure of intestine, while compound powder and Xiaoshi tablet could alleviate the damage. The old mice had stress response to high protein diet, which stimulated the activity of protein digestive enzyme to increase significantly, while the compound powder was gentle in stress response, significantly reduced the activity of protease, and showed a dose-dependent relationship. Therefore, the intestine damage of old mice by high beef protein diet could be improved by the compound powder. The compound powder can reduce the activity of protein digestive enzyme in high protein diet and the burden of the body. The mechanism of alleviating the activity of intestinal protease without reducing the digestibility of protein needs further study.
[1] 向运华,王晓慧.国内老年健康的研究现状与反思——基于CNKI的文献计量分析[J].华中科技大学学报(社会科学版),2019,33(5):20-27.
[2] 陈铮,李小鹰,杨爱民,等.关于《老年医院建设和管理标准》的建议[J].中华老年医学杂志,2018,37(10):1 065-1 068.
[3] 陈宪泽,汪婷婷,黄靖,等.老年群体健康管理与长期照护一体化模式研究[J].锦州医科大学学报(社会科学版), 2019,17(4):14-17.
[4] BAUER J M,BIOLO G,CEDERHOLM T,et al.Evidence-based recommendations for optimal dietary protein intake in older people:A position paper from the PROT-AGE study group[J].Journal of the American Medical Directors Association,2013,14(8):542-559.
[5] 中华人民共和国国家卫生和计划生育委员会.WS/T 556-2017老年人膳食指导[S].北京:中国标准出版社,2017.
[6] PEREIRA P M D C,VICENTE A F D R.Meat nutritional composition and nutritive role in the human diet[J].Meat Science,2013,93(3):586-592.
[7] 徐畅,阮榕生,刘玉环.浅述高蛋白饮食对人体健康的影响[J].食品工业科技,2012,33(21):399-402.
[8] 解玉怀,王丽雪,杨维仁,等.植物提取物对畜禽肠道免疫的影响及其调控机制[J].草业科学,2018,35(10):2 500-2 511.
[9] TAKUYA S. Regulation of intestinal epithelial permeability by tight junctions[J]. Cellular and Molecular Life Sciences, 2013, 4(70): 631-659.
[10] 燕宇婷,段亚男,王琦.益生菌改善肝性脑病肠道菌群、肠粘膜屏障功能的研究进展[J].世界最新医学信息文摘,2018,18(98): 86-89.
[11] CHEN H,CHENG H,LIU Y,et al.Konjac acts as a natural laxative by increasing stool bulk and improving colonic ecology in healthy adults[J].Nutrition,2006,22(11):1 112-1 119.
[12] STAIANO A, SIMEONE D, DEL GIUDICE E,et al.Effect of the dietary fiber glucomannan on chronic constipation in neurologically impaired children[J].The Journal of Pediatrics,2000,136(1):41-45.
[13] WANYO P,SIRIAMORNPUN S,MEESO N.Improvement of quality and antioxidant properties of dried mulberry leaves with combined far-infrared radiation and air convection in Thai tea process[J].Food and Bioproducts Processing,2011,89(1):22-30.
[14] GOFFREDO M,MASS K,PARKS E J,et al.Role of gut microbiota and short chain fatty acids in modulating energy harvest and fat partitioning in youth[J]. The Journal of Clinical Endocrinology and Metabolism,2016,101(11):4 367-4 376.
[15] 黄艳.桑叶粉/魔芋葡甘聚糖复配增强骨密度功效及肠道益生性研究[D].重庆:西南大学,2017.
[16] CHEN H L,CHENG H C,WU W T,et al.Supplementation of konjac glucomannan into a low-fiber Chinese diet promoted bowel movement and improved colonic ecology in constipated adults:A placebo-controlled,diet-controlled trial[J].Journal of the American College of Nutrition,2008,27(1):102-108.
[17] 钟耕,钟典.一种用于消除因食用火锅导致身体不适的食品及制备方法:中国,ZL 2014 1 0394527.7[P].2018-06-15.
[18] SONG S X,HOOIVELD G J,LI M J,et al.Dietary soy and meat proteins induce distinct physiological and gene expression changes in rats[J].Scientific Reports,2016,6:20 036.
[19] HE W S,WANG M G,PAN X X,et al.Role of plant stanol derivatives in the modulation of cholesterol metabolism and liver gene expression in mice[J].Food Chemistry,2013,140(1): 9-16.
[20] 秦清娟,邓利,徐小青,等.魔芋葡甘聚糖及其衍生物肠道益生性的体外发酵评价[J].食品科学,2015,36(15):217-220.
[21] 杨倩.动物组织学与胚胎学[M].北京:中国农业大学出版社,2008.
[22] 刘凡,李艳芳.断奶仔猪胃肠道免疫及营养研究[J].河南畜牧兽医(综合版),2019,40(3):9-11.
[23] 岳晓敬,扶雄锋,胡栾莎,等.复合益生菌发酵豆粕对断奶仔猪肠道形态和消化酶活性的影响[J].中国畜牧杂志, 2016,52(11):49-54.
[24] 王喆,袁希平,王安奎,等.牛品种、性别对高档牛肉粗蛋白和氨基酸含量的影响[J].云南农业大学学报(自然科学版),2011,26(5):633-638.
[25] ITO K,MATSUURA K,MIHARA Y,et al.Delivery of pancreatic digestive enzymes into the gastrointestinal tract by pancreatic exocrine tissue transplant[J].Scientific Reports,2019,9(1):5 922.
[26] LIN P H,SHIH B L,HSU J C.Effects of different sources of dietary non-starch polysaccharides on the growth performance,development of digestive tract and activities of pancreatic enzymes in goslings[J].British Poultry Science,2010,51(2):270-277.
[27] YANG Jing,YANG Lin,WANG Yongchang,et al.Effects of dietary protein and energy levels on digestive enzyme activities and electrolyte composition in the small intestinal fluid of geese[J].Animal Science Journal,2017,88(2):294-299.
[28] BRZEK P,BRZEK P,CIMINARI M E,et al.Effect of age and diet composition on activity of pancreatic enzymes in birds[J].Journal of Comparative Physiology B,2013,183(5):685-697.
[29] ZHAO F, HOU S S, ZHANG H F,et al.Effects of dietary metabolizable energy and crude protein content on the activities of digestive enzymes in jejunal fluid of Peking ducks[J].Poultry Science,2007,86(8):1 690-1 695.
[30] BASHIARDES S,TUGANBAEV T,FEDERICI S,et al.The microbiome in anti-cancer therapy[J].Seminars in Immunology,2017,32:74-81.
[31] 李爱杰.水产动物营养与饲料学[M].北京:中国农业出版社,1996:163-164.
[32] 邵庆均,苏小凤,许梓荣.饲料蛋白水平对宝石鲈增重和胃肠道消化酶活性影响[J].浙江大学学报(农业与生命科学版),2004,30(5):553-566.
