该文建立了高效液相色谱-二极管阵列检测器法测定马铃薯及其制品中的α-茄碱和α-卡茄碱的方法。样品经酸化甲醇提取后,用无水硫酸钠和无水乙酸镁吸收多余的水分后离心,用高效液相色谱-二极管阵列检测器法进行检测。检测条件:用Hubble C18液相色谱柱(250 mm×4.6 mm,5 μm),以乙腈和0.02 mol/L磷酸二氢钾溶液为流动相,流速为0.2 mL/min,柱温为30 ℃,进样量为10 μL,二级管阵列检测器检测范围190～810 nm,检测波长为195 nm。α-茄碱和α-卡茄碱在0.2～200 μg/mL时线性相关系数R2为0.999 3～0.999 8,方法检出限均为0.5 mg/kg,定量限均为1.7 mg/kg,4 、6、10 μg/mL三个浓度加标水平的回收率为98%～107%,相对标准偏差为0.05%～0.44%。该方法前处理简单、灵敏度高、重现性和回收率好,有良好的精密度、准确度,可用于马铃薯及其制品中α-茄碱和α-卡茄碱分析检测。
A method for the determination of α-solanine and α-chaconine in potato and its products by high performance liquid chromatography with diode array detector was established. After being extracted by acidified methanol, the sample was centrifuged with anhydrous sodium sulfate and anhydrous magnesium acetate to absorb the excess water. The sample was separated by Hubble C18 liquid chromatography column (250 mm× 4.6 mm, 5 μm). Acetonitrile and 0.02 mol/L potassium dihydrogen phosphate solution were used as the mobile phases. The flow rate was 0.2 mL/min, the column temperature was 30 ℃, the sample volume was 10 μL, and the detection range of the detector was 190-810 nm, the detection wavelength was 195 nm. The linear correlation coefficient r was 0.999 3-0.999 8 in the concentration range of 0.2-200 μg/mL, the detection limit of the method was 0.5 mg/kg, the quantitative limit was 1.7 mg/kg, the recoveries of 4, 6 and 10 μg/mL were 98%-107%, and the relative standard deviation was 0.05%-0.44%. The method has the advantages of simple pretreatment, high sensitivity, good reproducibility and recovery, good precision and accuracy, and could be used for the analysis and detection of α-solanine and α-chaconine in potato and its products.
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