综述与专题评论

基于核酸的分子生物学技术在食品过敏原检测中的应用进展

  • 苏琰 ,
  • 李融
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  • 1(合肥职业技术学院,安徽 合肥,238000)
    2(巢湖学院,安徽 合肥,238000)
硕士,副教授(本文通讯作者,E-mail:sy@htc.edu.cn)

收稿日期: 2019-11-04

  网络出版日期: 2020-07-15

基金资助

安徽省教育厅高校自然科学重点研究项目 (KJ2019A1125);安徽省教育厅青年拔尖人才项目(gxyq2017231);合肥职业技术学院校级科研机构(KYJG201806Z)

Application of molecular biological technology based on nucleic acid in food allergen analysis

  • SU Yan ,
  • LI Rong
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  • 1(Hefei Technology College, Hefei 238000, China)
    2(Chaohu University, Hefei 238000, China)

Received date: 2019-11-04

  Online published: 2020-07-15

摘要

食品过敏原是食物中包含的可能引发超敏反应性疾病的一类变应原。食品过敏原检测目前主要包括蛋白质检测与核酸检测两方面,基于核酸的分子生物学技术包括核酸扩增技术和杂交技术,能更加快捷、简便、高效地检测食品中的过敏原基因。该文简述了基于核酸的分子生物学检验技术的原理及方法类型,对近年来国内外建立的各类食品过敏原的核酸检测方法进行了综述,并将蛋白质检测技术与核酸检测技术的优缺点进行了比较,指出目前食品过敏原检测技术仍然存在的不足之处,以期为食品过敏原检测技术的进一步开发与研究提供参考。

本文引用格式

苏琰 , 李融 . 基于核酸的分子生物学技术在食品过敏原检测中的应用进展[J]. 食品与发酵工业, 2020 , 46(12) : 306 -311 . DOI: 10.13995/j.cnki.11-1802/ts.022704

Abstract

Food allergens are contained in food and may cause hypersensitivity. The detection technology of food allergens includes protein detection and nucleic acid detection. Molecular biological detection technology based on nucleic acid , which includes amplification techniques and hybridization techniques, detects allergen genes in food more quickly, easily and effectively. To provide references for the further development and research of food allergen detection technology, this paper briefly introduces the principles and methods of molecular biology technology based on nucleic acid, summarizes the nucleic acid detection methods for food allergens established at home and abroad in recent years, and compares the advantages and disadvantages of protein detection technology and nucleic acid detection technology so as to point out the shortcomings of the current food allergen detection technology.

参考文献

[1] HUBER B.100 jahre allergie:Clemens von pirquet-sein allergiebegriff und das ihm zugrunde liegende krankheitsverständnis[J].Wiener Klinische Wochenschrift,2006,118(19-20):573-579.
[2] 葛静静,刘秀梅,矫晓玲,等.婴儿早期不同程度过敏风险高危因素大样本调查[J].中国微生态学杂志,2019,31(7):820-824.
[3] 古淑青,赵超敏,程甲,等.基于质谱技术的食品过敏原检测方法研究进展[J].色谱,2016,34(7):639-646.
[4] Codex Alimentarius Commission.General standard for the labelling of prepackaged foods (codex stan 1-1985,amendment.7-2010)[S].2010.
[5] GB 7718—2011食品安全国家标准预包装食品标签通则[S].北京:中国标准出版社,2011.
[6] 日本内阁府.《食品标识基准》[S].2015-03-20.
[7] 闫瑞.消费者食品过敏原标签的认知现状与对策研究[D].太原:山西医科大学,2016.
[8] 王梦娟,李江华,郭林宇,等.欧盟食品中过敏原标识的管理及对我国的启示[J]. 食品科学,2014,35(1):273-277.
[9] MULLIS K B.The unusual origin of the polymerase chain reaction[J].Scientific American,1990,262(4):64-65.
[10] SAIKI R K,GELFAND D H,STOFFEL S,et al.Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase[J].Science (Washington D C),1988,239(4 839):487-491.
[11] 彭年才.数字PCR:原理、技术及应用[M].北京:科学出版社,2017.
[12] SU Yan,LI Rong.Development of dual priming oligonucleotide-polymerase chain reaction (DPO-PCR) for detection of wheat component in foods[J].Cellular and Molecular Biology,2019,65(6):80-84.
[13] 林佳琪,苏国成,苏文金,等.数字PCR技术及应用研究进展[J].生物工程学报,2017,33(2):170-177.
[14] YANO T,SAKAI Y,UCHIDA K,et al.Detection of walnut residues in processed foods by polymerase chain reaction[J].Bioscience Biotechnology and Biochemistry,2007,71(7):1 793-1 796.
[15] CHAMBERLAIN J S,GIBBS R A,RAINER J E,et al.Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification[J].Nucleic Acids Research,1988,16(23):11 141-11 156.
[16] 翟立公,杨剑婷,李永泉,等.德尔卑沙门氏菌血清型分子检测靶点筛选及多重PCR检测方法的建立[J].食品与发酵工业,2019,45(7):269-275.
[17] 梁华,王少辉,吴晓君,等.禽大肠杆菌、肠炎、鼠伤寒、鸡白痢及鸡伤寒沙门菌多重PCR方法的建立及应用[J].微生物学通报,2019,46(4):960-966.
[18] SUH S M,PARK S B,KIM M J,et al.Simultaneous detection of fruit allergen-coding genes in tomato,apple,peach and kiwi through multiplex PCR[J].Food Science and Biotechnology,2019,10(6):1-6.
[19] 王玮,韩建勋,吴亚君,等.芥末等8种食物过敏原的多重PCR检测技术[J].食品与发酵工业,2011,37(6):156-160.
[20] 王玮,韩建勋,袁飞,等.多重PCR同时检测常见8种食物过敏原[J].中国食品学报,2011,11(6):152-157.
[21] CHENG Fang,WU Jiajie,ZHANG Jin,et al.Development and inter-laboratory transfer of a decaplex polymerase chain reaction assay combined with capillary electrophoresis for the simultaneous detection of ten food allergens[J].Food Chemistry,2016,199:799-808.
[22] 周丽军.副猪嗜血杆菌及巴氏杆菌双重荧光定量PCR检测方法的建立[J].中国预防兽医学报,2019,41(5):489-494.
[23] EISCHEID A C,STADIG S R.A group-specific,quantitative real-time PCR assay for detection of crab,a crustacean shellfish allergen,in complex food matrices[J].Food Chemistry,2018,244:224-231.
[24] 孙良广,黄文婧.实时荧光PCR技术快速检测莲蓉制品中芸豆成分[J].食品科学,2017,38(22):337-341.
[25] LOPEZ-CALLEJA I M,CRUZ S D L,GONZALEA I,et al.Market analysis of food products for detection of allergenic walnut (Juglans regia) and pecan (Carya illinoinensis) by real-time PCR[J].Food Chemistry,2015,177:111-119.
[26] LINACERO R,BALLESTEROS I,SANCHIZ A,et al.Detection by real timePCR of walnut allergen coding sequences in processed foods[J].Food Chemistry,2016,202:334-340.
[27] LOPEZ-CALLEJA I M,CRUZ S D L,PEGELS N,et al.Sensitive and specific detection of almond (Prunus dulcis) in commercial food products by real-time PCR[J].LWT-Food Science and Technology,2014,56(1):31-39.
[28] SANCHIZ A,BALLESTROS I,MARQUES E,et al.Evaluation of locked nucleic acid and TaqMan probes for specific detection of cashew nut in processed food by real time PCR[J].Food Control,2018,89:227-234.
[29] KIM M J,KIM H I,KIM J H,et al.Rapid on-site detection of shrimp allergen tropomyosin using a novel ultrafast PCR system[J].Food Science and Biotechnology,2019,28(2):591-597.
[30] GRAZIANO S,GULLI M,MARMIROLI N.Detection of allergen coding sequences of kiwi,peach,and apple in processed food by qPCR.[J].J Sci Food Agric,2017,98(8):3 129-3 139.
[31] 汪永信,程潇,安虹,等.实时荧光PCR法同时检测食物中大豆和芹菜致敏原成分[J].生物技术通报,2016,32(1):69-73.
[32] GARCIA A,MADRID R,GARCIA T,et al.Detection of food allergens by taqman real-time PCR methodology[J].Methods Mol Biol,2017,1 592:95-108.
[33] 尚柯,张彪,段庆梓,等.小麦和榛子过敏原成分检测的实时荧光PCR方法[J].食品与发酵工业,2018,44(4):234-240.
[34] VOGELSTEIN B,KINZLER K W.Digital PCR[J].Proceedings of the National Academy of Sciences of the United States of America,1999,96(16):9 236-9 241.
[35] 冯兆民,舒跃龙.数字PCR技术及其应用进展[J].病毒学报,2017,33(1):103-107.
[36] KOPPEL R,GANESHAN A,WEBER S,et al.Duplex digital PCR for the determination of meat proportions of sausages containing meat from chicken,turkey,horse,cow,pig and sheep[J].European Food Research and Technology,2019,245(4):853-862.
[37] 缪青梅,汪小福,陈笑芸,等.基于双重微滴数字PCR精准定量转基因水稻G6H1的方法研究[J].农业生物技术学报,2019,27(1):163-173.
[38] PIERBONI E,RONDINI C,TORRICELLI M,et al.Digital PCR for analysis of peanut and soybean allergens in foods[J].Food Control,2018,92:128-136.
[39] MAYER W,SCHULLER M,VIEHAUSER M C,et al.Quantification of the allergen soy (Glycine max) in food using digital droplet PCR (ddPCR)[J].European Food Research and Technology,2018,245(2):499-509.
[40] NOTOMI T.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Res,2000,28(12):E63.
[41] NAGAMINE K,HASE T,NOTOMI T.Accelerated reaction by loop-mediated isothermal amplification using loop primers[J].Mol Cell Probes,2002,16(3):223-229.
[42] YANG Q, DOMESLE K J, GE B. Loop-mediated isothermal amplification for salmonella detection in food and feed: Current applications and future directions[J].Foodborne Pathogens and Disease,2018,15(6):309-331.
[43] ANANDAKUMAR L,BAGYALAKSHMI K,NITHYA K,et al.Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid diagnosis of sugarcane yellow leaf virus in sugarcane[J].Sugar Tech,2018,20(6):708-716.
[44] 赵远洋,王瑾,林丽萍,等.基于实时荧光环介导等温扩增快速检测鸡肉中的大肠杆菌O157:H[J].中国食品学报,2019,19(3):287-294.
[45] YUAN Dan,KONG Jilie,LI Xinxin,et al.Colorimetric LAMP microfluidic chip for detecting three allergens:Peanut,sesame and soybean[J].Scientific Reports,2018,8(1):8 682-8 688.
[46] MAO Ruifeng,XIE Kaiwen,ZHAO Menghuan,et al.Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of pistachio (pistacia vera) in food samples[J].Food Analytical Methods,2020,13:658-666.
[47] SUN Min,GAO Hongwei,XIAO Xizhi,et al.A novel loop-mediated isothermal amplification method for detection of the carrot materials in foods[J].European Food Research and Technology,2015,241(2):295-302.
[48] 蒋静,谌鸿超,樊彦莉,等.小麦成分环介导等温扩增现场快速检测方法的建立和应用[J].食品安全质量检测学报,2017,8(4):103-109.
[49] 刘津,张隽,李婷,等.食品过敏原澳洲坚果环介导等温扩增检测方法的建立与应用[J].食品科学,2014,35(22):226-232.
[50] 程晋霞,周熙诚,马丹,等.食品中芥末过敏原成分LAMP检测方法的建立[J].食品科学,2014,35(20):148-152.
[51] 马新秀,胡文忠,冯可,等.生物芯片在微生物检测中的应用[J].食品与发酵工业,2018,44(2):273-277.
[52] CHRISTOPOULOU S,KARAISKOU S,KALOGIANNI D P.Microbead-based simultaneous fluorometric detection of three nut allergens[J].Microchimica Acta,2018,185(13):1-8.
[53] PASQUARELLI A.Biochips:Technologies and applications[J].Materials Science & Engineering C,2008,28(4):495-508.
[54] ABI A,MOHAMMADPOUR Z,ZUO X,et al.Nucleic acid-based electrochemical nanobiosensors[J].Biosensors & Bioelectronics,2018,102:479-489.
[55] WANG Wei,LI Yuanyuan,ZHAO Fangyuan,et al.Optical thin-film biochips for multiplex detection of eight allergens in food [J].Food Research International,2011,44(10):3 229-3 234.
[56] WANG Wei,HAN Jianxun,WU Yajun,et al.Simultaneous detection of eight food allergens using optical thinfilm biosensor chips[J].Journal of Agricultural & Food Chemistry,2011,59(13):6 889-6 894.
[57] SUN Xiulan,JIA Min,GUAN Lu,et al.Multilayer graphene-gold nanocomposite modified stem-loop DNA biosensor for peanut allergen-Ara h1 detection[J].Food Chemistry,2015,172:335-342.
[58] MOMTIEL V R V,TORRENTE-RODRIGUEZ R M,RIVERA G G D,et al.Amperometric determination of hazelnut traces by means of Express PCR coupled to magnetic beads assembled on disposable DNA sensing scaffolds[J].Sensors and Actuators B:Chemical,2017,245:895-902.
[59] TOTAJADA-GENARO L A,SANTIAGO-FELIPE S,MORAIS S,et al.Multiplex DNA detection of food allergens on a digital versatile disk[J].Journal of Agricultural and Food Chemistry,2012,60(1):36-43.
[60] 孟佳,古淑青,方真,等.高效液相色谱-串联质谱法测定肉制品和调味料中7种水产品过敏原[J].色谱,2019,37(7):712-722.
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