该文旨在研究余甘子不同溶剂提取物体外抗氧化活性和对H2O2诱导RAW264.7细胞损伤的保护作用。结果表明,余甘子不同溶剂提取物能有效清除超氧阴离子自由基和羟自由基,具有良好还原能力和总抗氧化能力,一定浓度下抗氧化效果与抗坏血酸相当。余甘子不同溶剂提取物能有效提高细胞内超氧化物歧化酶活力、乳酸脱氢酶活力及谷胱甘肽含量,降低丙二醛值,剂量依赖性保护氧化损伤的RAW264.7细胞。其中,余甘子70%(体积分数)乙醇提取物表现出最佳体外抗氧化活性和对氧化损伤细胞的保护作用,这与其富含没食子酸、柯里拉京、鞣花酸及槲皮素有关。总之,余甘子不同溶剂提取物能有效改善H2O2诱导的细胞损伤,其作用机制可能与清除自由基和提高抗氧化酶活性有关。该研究为开发余甘子功能食品或保健品提供研究依据。
The purpose of this paper was to study antioxidant activity and protective effect of H2O2-induced damage in RAW264.7 cell treatment with different solvents extracts from Phyllanthus emblica(DEPE). The results showed that DEPE could effectively scavenge superoxide anions and hydroxyl radicals, and had strong reducing power and total antioxidant capacity. DEPE could protect RAW264.7 cells from oxidative damage at a dose-dependent manner by increasing intracellular superoxide dismutase activity, lactate dehydrogenase activity and glutathione content, and reducing malondialdehyde value. Among them, 70% ethanol extract from Phyllanthus emblica showed the best in-vitro antioxidant activity and protective effect on oxidatively damaged cells, which was related to its rich components including gallic acid, corilagin, ellagic acid and quercetin. In conclusion, DEPE could protect RAW264.7 cells from damage which was induced by H2O2, and its mechanism might be related to scavenge free radicals and increase antioxidant enzyme activity. This study provided a research basis for the development of functional foods or health products of Phyllanthus emblica.
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