食品与发酵工业 >

2020 , Vol. 46 >Issue 16: 226 - 230

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023311

分析与检测

基于荧光共振能量转移的免疫传感器检测黄曲霉毒素B1

  • 欧阳秀酝 ,
  • 李琳 ,
  • 张林威 ,
  • 程云辉 ,
  • 许宙
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  • 1 长沙理工大学 化学与食品工程学院,湖南 长沙,410114
    2 华南理工大学 食品科学与工程学院,广东 广州,511442
硕士研究生(许宙副教授为通讯作者,E-mail: xz_jnu@126.com)

收稿日期: 2020-01-09

  修回日期: 2020-02-19

  网络出版日期: 2020-09-17

基金资助

国家重点研发计划(2016YFF0203800;2016YFF0103701);国家自然科学基金(31401566);湖南省创新平台与人才计划(2017RS3055);粮食深加工与品质控制湖南省2011协同创新项目(2013448);常熟市科技发展计划(社会发展类)项目(CS201605);苏州市科技计划项目(SNG201617)

收起

Detection of aflatoxin B1 by immunosensor based on fluorescence resonance energy transfer

  • OUYANG Xiuyun ,
  • LI Lin ,
  • ZHANG Linwei ,
  • CHENG Yunhui ,
  • XU Zhou
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  • 1 School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, China
    2 School of Food Science and Engineering, South China University of Technology, Guangzhou 511442, China

Received date: 2020-01-09

  Revised date: 2020-02-19

  Online published: 2020-09-17

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摘要

制备水溶性上转换荧光纳米颗粒(up-conversion nanoparticles, UCNPs),表面修饰黄曲霉毒素B1(aflatoxin B1, AFB1)抗原作为能量供体探针;在金纳米颗粒(gold nanoparticles, AuNPs)表面修饰AFB1抗体作为能量受体探针,构建了一种基于荧光共振能量转移(fluorescence resonance energy transfer, FRET)的免疫传感器用于检测AFB1。结果表明,该检测体系中在AFB1质量浓度0.05~20 ng/mL线性相关(R2=0.990 9),检测限为0.02 ng/mL。将该方法应用于花生油样品中AFB1的检测,当加标水平为0.1、1、10 ng/mL时,回收率为106%~112%,相对标准偏差低于4.7%。该方法具有高特异性和高灵敏度的特点,可用于食品中AFB1的快速定量检测。

关键词： 荧光共振能量转移; 上转换荧光纳米颗粒; 金纳米颗粒; 黄曲霉毒素B1; 抗原; 抗体

本文引用格式

欧阳秀酝 , 李琳 , 张林威 , 程云辉 , 许宙 . 基于荧光共振能量转移的免疫传感器检测黄曲霉毒素B1[J]. 食品与发酵工业, 2020 , 46(16) : 226 -230 . DOI: 10.13995/j.cnki.11-1802/ts.023311

Abstract

With water-soluble up-converting fluorescent nanoparticles (UCNPs) and gold nanoparticles (AuNPs) as energy donor-receptor pairs, a fluorescence resonance energy transfer (FRET) immunosensor was designed for the detection of aflatoxin B1 (AFB1). The proposed FRET immunosensor revealed a wide linear range of AFB1 concentration in 0.05-20 ng/mL (R2=0.990 9) with an extremely low detection limit of 0.02 ng/mL. This method was applied to the detection of peanut oil samples free of AFB1 contamination. The recoveries of AFB1 at three spiked levels (0.1, 1, 10 ng/mL) were in the range of 106%-112% with the relative standard deviations (RSDs) less than 4.7%. These results showed that the FRET immunosensor established in this study had good specificity and high sensitivity in the rapid detection of AFB1 in food.

Key words： fluorescence resonance energy transfer; up-conversion nanoparticles; gold nanoparticles; aflatoxin B1; antigen; antibody

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