为了简化重组大肠杆菌生产番茄红素的工艺,该研究通过选择大肠杆菌中17个转录因子相关基因的启动子序列,以编码番茄红素合成基因的CrtEBI为目的基因,通过构建表达番茄红素合成基因的质粒,来评估转录因子相关基因启动子序列对番茄红素产量的影响。通过比较不同菌株合成番茄红素的能力,确定最佳启动子。结果表明,含有组成型启动子Plpp或Pmglb的菌株合成番茄红素的能力较强,相较于传统诱导型启动子菌株产量分别提高了347.7%和51.3%。由于筛选到的启动子为组成型启动子,省去了添加诱导剂的过程,达到了优化生产工艺、降低生产成本的目的。
Lycopene is a kind of fat-soluble carotenoids with strong antioxidant properties. It is widely used in food, medicine and other industries. Previous studies have constructed engineered Escherichia coli with high-level production of lycopene using inducer. But these methods are labor intensive and time-consuming. In order to simplify the lycopene production, 17 promoters that regulated by transcription factor in E. coli were selected and E. coli recombinants were constructed e by cloning promoter sequences and ligating with report gene crtEBI. E. coli recombinant with the promoter Plpp and Pmglb were mediated more effective expression. The lycopene yield increased by 51.3% compared with the control strain pTRC99aEBI.
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