建立测定饮料、配制酒、干制豆制品中胭脂红、日落黄、诱惑红、亮蓝、酸性红、碱性橙2、碱性橙21、碱性橙22、酸性橙2、碱性嫩黄O的高效液相色谱法。以V(甲醇)∶V(水)=8∶2为萃取溶剂对样品中色素直接萃取,高效液相色谱仪配二极管阵列检测器进行测定,色谱柱Agela Venusil XBP-C18(250 mm×4.6 mm, 5 μm)进 行分离;449、485、254、628 nm为最佳吸收波长;柱温25℃;流速1 mL/min;流动相20 mmol/L醋酸铵溶液-甲醇进行洗脱。经方法学验证,色谱峰分离度均＞1.5,在线性范围0.5~125 mg/kg内相关系数均＞0.999,液体样品加标回收率均＞85%,固体样品加标回收率均＞75%。以饮料为基质进行加标,计算得出10种色素的日内精密度为0.76%~4.10%(n=6),日间精密度为1.09%~5.29% (n=6)。结果表明,该方法准确性、灵敏度、精密度良好,可满足食品检测行业对其检测的要求。
The detection method of carmine, sunset yellow, allure red, brilliant blue, acid red, basic orange 2, basic orange 21, basic orange 22, acid orange 2, auramine O in beverages, prepared wines, and dried soy products O using high performance liquid chromatography were established. Methanol: water (8∶2) were used as the extraction solvent to directly extract the pigment from the sample. The high-performance liquid chromatograph equipped with a diode array detector for the determination and the column was Agela Venusil XBP-C18 XBP-C18 (250 mm×4.6 mm, 5 μm). The following condition was used in this study including 449 nm, 485 nm, 254 nm, and 628 nm absorption wavelengths; column temperature at 25 °C with 1 mL/min flow rate. Mobile phase: 20 mmol/L ammonium acetate solution-methanol for elution. According to the methodological verification, all the chromatographic peak resolutions were>1.5. The correlation coefficients within the linear range of 0.5 mg/kg-125 mg/kg were all> 0.999; the recovery rate of the liquid samples was > 85% and the recovery rate of the solid samples was > 75%. Based on the calculation of the standard addition of beverages, the intra-day precision (RSD%) of 10 pigments was 0.76%-4.10% (n=6), and the intra-day precision (RSD%) was 1.09%-5.29% (n=6). The results showed that the method had good accuracy, sensitivity and precision, and could meet the testing requirements of the food testing industry.
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