研究辣木叶多酚提取物(Moringa oleifera leaves extracts,MLE)对紫外线(ultraviolet,UV)照射引起的光老化小鼠皮肤的保护作用。分析了MLE对光老化皮肤中羟脯氨酸(hydroxyproline,HYP)、透明质酸(hyaluronic acid,HA)、氧化应激、基质金属蛋白酶(matrix metalloproteinases,MMPs)及形态结构的影响。结果表明,MLE显著提升了光老化皮肤中HYP和HA的含量(P<0.05)。UV照射对皮肤产生了显著的氧化应激,MLE能够提升皮肤中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和过氧化氢酶(catalase,CAT)的活性,以及谷胱甘肽(glutathione,GSH)的含量(P<0.05或P<0.01),降低了丙二醛(malondialdehyde,MDA)的含量(P<0.01),说明MLE能够调节光老化皮肤的氧化应激。同时,UV照射极显著的提升了皮肤中MMPs(MMP-1、MMP-3和MMP-9)的含量(P<0.01),MLE能够降低光老化皮肤中MMPs的含量(P<0.05或P<0.01)。此外,MLE能够有效地改善光老化皮肤中组织形态和胶原结构。MLE对光老化小鼠皮肤的保护作用可能与其多酚物质的抗氧化和抗炎活性相关。
This study aimed to evaluate the protective effect of Moringa oleifera leaves polyphenol extract (MLE) on ultraviolet (UV)-induced damages to mice skin. The effects of MLE on hydroxyproline (HYP), hyaluronic acid (HA), oxidative stress, matrix metalloproteinases (MMPs), and morphology in the photoaging skin of mice were analyzed. The results showed that MLE could significantly increase the contents of HYP and HA in the photoaging skin (P<0.05). While UV radiation could significantly increase oxidative stress in mice skin, MLE increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and the content of glutathione (GSH) of the photoaging skin (P<0.05 or P<0.01), and also MLE significantly decreased the content of malondialdehyde (MDA) of the photoaging skin (P<0.01), which indicated that MLE could regulate oxidative stress of the photoaging skin. Additionally, UV radiation significantly increased the content of MMPs (MMP-1, MMP-3 and MMP-9) of the photoaging skin (P<0.01), while MLE reduced the content of MMPs (MMP-1, MMP-3 and MMP-9) of the photoaging skin (P<0.05 or P<0.01). Furthermore, MLE could effectively improve the tissue morphology and collagen structure of the photoaging skin. In conclusion, the protective effect of MLE on the photoaging mice skin might be closely related to the anti-oxidative and anti-inflammatory activities of its polyphenols.
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