为改善美国大口胭脂鱼的腥味问题,采用红茶脱腥法对其脱腥,使用响应面软件优化设计美国大口胭脂鱼脱腥工艺,采用整体材料吸附萃取(monolithic material sorptive extraction,MMSE)提取美国大口胭脂鱼肉中的挥发性物质,并利用气相色谱-质谱-嗅闻联用法(gas chromatography-mass spectrometry-olfactometry,GC-MS-O)对胭脂鱼肉中的挥发性成分进行分析。用红茶对美国大口胭脂鱼进行脱腥处理,经响应面软件分析得到胭脂鱼的最优脱腥条件为:红茶用量为15 g/L,脱腥时间为41 min,脱腥温度为43 ℃。通过比较,脱腥后的挥发性物质相对百分含量都有所减少,鱼肉腥味明显减弱,其中,己醛,1-戊烯-3-醇和1-辛烯-3-醇,辛醛和壬醛等物质相对含量变化明显。
In order to improve the fishy smell of American bigmouth bass, black tea deodorization method was used for the deodorization. Response surface software was used to optimize the design of American bigmouth bass deodorization process. And volatile components in the American bigmouth bass were extracted by monolithic material sorptive extraction (MMSE), and volatile components in American bigmouth bass were analyzed by gas chromatography-mass spectrometry-olfactomtry(GC-MS-O). The results showed that the best conditions for American bigmouth bass deodorization were under the condition of soaking at 15 g/L black tea at 43 °C for 41 min. It was found that the relative percentages of volatile substances after deodorization was basically reduced, and the fishy smell was significantly reduced. The relative content of hexanal, 1-pentene-3-ol, and 1-octene-3-ol. and nonanal etc. also considerably changed.
[1] 褚新梅.美国大口胭脂鱼北方地区养殖适应性研究[J].现代农村科技,2015(5):43.
[2] YARNPAKDEE S,BENJAKUL S,NALINANON S,et al. Lipid oxidation and fishy odour development in protein hydrolysate from Nile tilapia(Oreochromis niloticus) muscle as affected by freshness and antioxidants[J]. Food Chemistry,2012,132(4):1 781-1 788.
[3] 王国超,李来好,郝淑贤,等. 水产品腥味物质形成机理及相关检测分析技术的研究进展[J].食品工业科技,2012(5):401-404,409.
[4] 卢祺,刘津延,刘方芳, 等.鱼类腥味物质及脱腥技术研究进展[J].食品工业科技技,2019,40(8):285-291.
[5] 王文勇,张英慧,赖长生.水产品腥味物质去除及提取分析的研究进展[J].肉类工业,2017(3):50-53.
[6] 陈漪,庄晶晶,尚艳丽,等.金枪鱼鱼肉茶水脱腥条件的比较研究[J].粮油食品科技,2012,20(6): 76-79
[7] 徐永霞,姜程程,刘滢,等.带鱼脱腥工艺及脱腥前后的理化性质[J].食品与发酵工业,2013,39(12): 68-72.
[8] 钱攀,马旭婷,许刚,等.美国鲢鱼挥发性成分和脱腥方法研究[J].中国食品学报,2016,16(12):169-176.
[9] 葛胜晗,周阿容,黄晨楹, 等.红茶对海螺肉去腥和保鲜作用的研究[J].中国调味品,2018,43(12):23-31.
[10] 苏怡,姜启兴,夏文水.不同脱腥方法对鲟鱼肉脱腥效果的比较研究[J].食品科技,2019,44(10):138-146.
[11] 吴燕燕,王悦齐,李来好,等.基于电子鼻与HS-SPME-GC-MS技术分析不同处理方式腌干带鱼挥发性风味成分[J].水产学报,2016,40(12):1 931-1 940.
[12] 刘玉平,陈海涛,孙宝国. 鱼肉中挥发性成分提取与分析的研究进展[J]. 食品科学,2009,30(23):447-451.
[13] 赵亮, 马凌云. GC-MS法分析南湾鳙鱼鱼肉挥发性成分的组成[J].食品与机械, 2011, 27(6): 80-82.
[14] BENZO M, GILARDONI G, GANDINI C, etal.Determination of the threshold odor concentration of main odorants in essential oils using gas chromatography-olfactometry incremental dilution technique[J].Journal of ChromatographyA, 2007, 1 150(1-2):131-135.
[15] 郑平安,黄健,孙静,等. HS-SPME结合GC-MS法分析鲐鱼肉加热前后挥发性成分变化[J].食品科学,2012,33(14):242-246.
[16] MOHAMMAD A M, AMURADHA B, HITOSHI T, et al. Volatile flavor compounds of some sea fish and prawn species[J]. Fisheries Science, 2003, 69(4): 864-866.
[17] ALICIA O, JOSELUIS N, MONICA F. Establishment of the contribution of volatile compounds to the aroma of fermentedsausages at different stages of processing and storage[J]. Food Chemistry, 2009, 115(4):1 464-1 472.
[18] JOSEPHSON D B,LINDSAY R C,STUIBER D A. Enzymic hydroperoxide initiated effects in fresh fish[J].Food Sci,1985, 52: 596-600.
[19] JOSEPHSON D B, LINDSAY R C. Volatile compounds characterizing the aroma of fresh atlantic and pacific oysters[J]. J Food Sci, 1987,50: 5-9.
[20] XIE Jianchun, SUN Baoguo, ZHENG Fuping, et al. Volatile flavorcompounds in roasted pork of Mini-pig[J]. Food Chemistry, 2008,109(3): 506-514.
[21] SERKAN SELLI,CAROLE PROST B, THIERRY SEROT.Odour-active and off-odour components in rainbow trout (Oncorhynchus mykiss) extracts obtained by microwave assisted distillation-solvent extraction[J]Food Chemistry,2008, 114(1):317-322.
[22] 杨倩倩,邱杨,余以刚,等. 养殖大黄鱼脱脂脱腥处理前后挥发性成分的变化[J].食品科学,2012,33(14):206-210.