食品与发酵工业 >

2020 , Vol. 46 >Issue 18: 192 - 196

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.022766

生产与科研应用

椰子油降解菌的筛选及降解条件优化

  • 黄怀兴 ,
  • 邓毛程 ,
  • 李静 ,
  • 吴林杰 ,
  • 蔡亮 ,
  • 王富程 ,
  • 黄洁华 ,
  • 林铿淳 ,
  • 廖民聪
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  • (广东轻工职业技术学院 食品与生物技术学院,广东 广州,510300)
大专生(邓毛程教授和李静教授为共同通讯作者,E-mail:dengmc@163.com;eeslee2005@163.com)

收稿日期: 2019-11-10

  修回日期: 2020-06-05

  网络出版日期: 2020-10-23

基金资助

珠江学者人才项目(KYRC2007-001);2019年广东省普通高校特色创新项目(2019GKTSCX011);2016年特支计划人才项目(KYRC2007-003);广东轻工职业技术学院第十八届挑战杯项目 (2019-A-25);2019年广东轻工职业技术学院创新创业训练项目(1083319003)

收起

Screening and degradation conditions of coconut oil-degrading strain

  • HUANG Huaixing ,
  • DENG Maocheng ,
  • LI Jing ,
  • WU Linjie ,
  • CAI Liang ,
  • WANG Fucheng ,
  • HUANG Jiehua ,
  • LIN Kengchun ,
  • LIAO Mincong
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  • (College of Food and Biotechnology Technology,Guangdong Industry Polytechnic, Guangzhou 510300, China)

Received date: 2019-11-10

  Revised date: 2020-06-05

  Online published: 2020-10-23

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摘要

为了对椰蓉进行发酵法脱脂,该文筛选椰子油高效菌种并研究该菌种的降解条件。以自然发酵椰浆为原料,筛选获得1株椰子油高效降解菌株YD22。经菌体形态与分子生物学鉴定,该菌株被鉴定为解脂亚罗酵母(Yarrowia lipolytica)。通过氮源与外加碳源的优选试验,分别确定酵母膏为最佳氮源,柠檬酸钠为最佳的外加碳源,它们的最佳用量分别为10 g/L和7.5 g/L。结果表明,在含有质量浓度为40 g/L椰子油的最佳培养基中,解脂亚罗酵母YD22发酵120 h,椰子油降解率达到85.76%,该研究结果可为椰蓉发酵法脱脂提供参考。

关键词： 椰蓉; 椰子油; 发酵法脱脂; 降解; 菌种筛选

本文引用格式

黄怀兴 , 邓毛程 , 李静 , 吴林杰 , 蔡亮 , 王富程 , 黄洁华 , 林铿淳 , 廖民聪 . 椰子油降解菌的筛选及降解条件优化[J]. 食品与发酵工业, 2020 , 46(18) : 192 -196 . DOI: 10.13995/j.cnki.11-1802/ts.022766

Abstract

To remove coconut oil from the desiccated coconut by microbial fermentation, the highly effective coconut oil-degrading strains were isolated, and the degradation conditions of the target strain were optimized. Using natural fermented coconut milk as culture substance, strain YD22 was screened out with the highest degradation efficiency of coconut oil. It was determined as Yarrowia lipolytica by morphology and molecular identification. Through optimal experiments, yeast extract and sodium citrate were determined as the best nitrogen source and appending carbon source. Furthermore, the optimal dosage of them was 10 g/L and 7.5 g/L, respectively. Under the optimal conditions, the degradation rate of culture medium containing 40 g/L coconut oil reached 85.76% by YD22 after 120 h of incubation. The result could provide references for degreasing of desiccated coconut by fermentation.

Key words： desiccated coconut; coconut oil; degrease by fermentation; degradation; strain screening

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