为了对椰蓉进行发酵法脱脂,该文筛选椰子油高效菌种并研究该菌种的降解条件。以自然发酵椰浆为原料,筛选获得1株椰子油高效降解菌株YD22。经菌体形态与分子生物学鉴定,该菌株被鉴定为解脂亚罗酵母(Yarrowia lipolytica)。通过氮源与外加碳源的优选试验,分别确定酵母膏为最佳氮源,柠檬酸钠为最佳的外加碳源,它们的最佳用量分别为10 g/L和7.5 g/L。结果表明,在含有质量浓度为40 g/L椰子油的最佳培养基中,解脂亚罗酵母YD22发酵120 h,椰子油降解率达到85.76%,该研究结果可为椰蓉发酵法脱脂提供参考。
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