食品与发酵工业 >

2020 , Vol. 46 >Issue 18: 197 - 202

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023024

分析与检测

液相色谱-串联质谱法测定木瓜发酵饮料中的三种α-二羰基化合物

  • 陈荣祥 ,
  • 顾雯 ,
  • 罗敏 ,
  • 何婷 ,
  • 尹考 ,
  • 罗春燕
展开
  • 1(遵义医科大学,分析测试中心,贵州 遵义,563000)
    2(遵义医科大学 药学院,贵州 遵义,563000)
    3(遵义医科大学 检验医学院,贵州 遵义,563000)
博士,副教授(本文通讯作者,E-mail: chenrongxiang2014@163.com)

收稿日期: 2019-12-09

  修回日期: 2020-02-12

  网络出版日期: 2020-10-23

基金资助

国家自然科学基金项目(81760652);贵州省科技厅基础研究项目(黔科合基础[2016] 1172);波谱与原子分子物理国家重点实验室开放课题(T151601);遵义医科大学大学生创新创业项目(201751092;ZYDC2018029;2018103)

收起

Determination of three α-dicarbonyl compounds in Fructus chaenomelis fermented beverage by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)

  • CHEN Rongxiang ,
  • GU Wen ,
  • LUO Min ,
  • HE Ting ,
  • YIN Kao ,
  • LUO Chunyan
Expand
  • 1(Analytical and Testing Center, Zunyi Medical University, Zunyi 563000, China)
    2(School of Pharmaceutical Sciences, Zunyi Medical University, Zunyi 563000, China)
    3(School of Laboratory Medicine, Zunyi Medical University, Zunyi 563000, China)

Received date: 2019-12-09

  Revised date: 2020-02-12

  Online published: 2020-10-23

Fold

摘要

该文采用邻苯二胺柱前衍生化,建立了超高效液相色谱-串联质谱法测定木瓜发酵饮料中乙二醛、丙酮醛和丁二酮3种α-二羰基化合物含量的方法。样品加水稀释后采用离心超滤进行净化,室温下衍生,衍生产物采用Waters XBridge BEH C18色谱柱(100 mm×2.1 mm,1.7 μm)分离,流动相为乙腈-0.2%(体积分数)乙酸溶液,梯度洗脱,采用电喷雾离子源以正离子多重反应监测模式进行质谱检测。结果表明,3种α-二羰基化合物在0.012~0.900 mg/L线性关系良好,相关系数(r2)均>0.99,平均加样回收率在86.26%～95.29%,RSD为2.49%～7.31%(n=6)。该方法快速灵敏,适合于木瓜发酵饮料中α-二羰基化合物的检测。

关键词： 超高效液相色谱; 串联质谱法; α-二羰基化合物; 乙二醛; 丙酮醛; 丁二酮

本文引用格式

陈荣祥 , 顾雯 , 罗敏 , 何婷 , 尹考 , 罗春燕 . 液相色谱-串联质谱法测定木瓜发酵饮料中的三种α-二羰基化合物[J]. 食品与发酵工业, 2020 , 46(18) : 197 -202 . DOI: 10.13995/j.cnki.11-1802/ts.023024

Abstract

A method for the determination of glyoxal, methylglyoxal and dimethylglyoxal in Fructus chaenomelis fermented beverage by UPLC-MS/MS with o-phenylenediamine pre-column derivatization was established. The sample was diluted with water and purified by centrifugal ultrafiltration method. The derivative products were separated on a Waters XBridge BEH C18 column (100 mm×2.1 mm, 1.7 μm) with a gradient elution system, the mobile phase was acetonitrile-0.2% acetic acid solution. Eluted compounds were detected in the electrospray ionization positive mode with multiple reaction monitoring. The results showed that the linear relationship of these three α-dicarbonyl compounds were good in the range of 0.012-0.900 mg/L, the correlation coefficient (r2) were greater than 0.99. The average recovery was 86.26%-95.29% with the relative standard deviation (RSD) of 2.49%-7.31% (n=6). This method is rapid, sensitive and thus could be successfully applied to the analysis of α-dicarbonyl compounds in fermented beverage.

Key words： ultra performance liquid chromatography; tandem mass spectrometry; α-dicarbonyl compounds; glyoxal; methylglyoxal; dimethylglyoxal

参考文献

[1] 尹震花, 赵晨, 张娟娟,等. 光皮木瓜的化学成分及药理活性研究进展[J].中国实验方剂学杂志, 2017,23(9): 221-229.
[2] 淳泽利, 陈荣祥, 保玉心. OPA柱前衍生反相高效液相色谱法测定野木瓜发酵饮料中的游离氨基酸[J].现代农业科技, 2019(4): 217-218;221.
[3] 王瑜, 李立郎, 张洁,等. 野木瓜酵素发酵过程中活性成分的变化[J].现代食品科技, 2019,35(10): 125-130.
[4] 黄启瑞, 曾茂茂, 何志勇,等. 反应条件对葡萄糖-甘氨酸模拟美拉德反应体系中α-二羰基化合物生成的影响[J].食品工业科技, 2016,37(8): 150-154.
[5] PAPETTI A, MASCHERPA D, GAZZANI G. Free alpha-dicarbonyl compounds in coffee, barley coffee and soy sauce and effects of in vitro digestion[J].Food Chemistry, 2014,164: 259-265.
[6] 陈燕玲, 罗婷, 曾洪敏,等. 丙酮醛对人近曲小管上皮细胞氧化应激状态及Nlrp3炎症小体信号的作用[J].医学研究生学报, 2018,31(3): 262-266.
[7] HELLWIG M, GENSBERGER-REIGL S, HENLE T, et al. Food-derived 1,2-dicarbonyl compounds and their role in diseases[J].Seminars in Cancer Biology, 2018,49: 1-8.
[8] 蔡智立, 陈诗炜, 陈媛媛,等. 食品中α-二羰基化合物产生的危害及控制[J].中国调味品, 2018,43(10): 190-194.
[9] HENNING C, GLOMB M A. Pathways of the maillard reaction under physiological conditions[J].Glycoconjugate Journal, 2016,33(4): 499-512.
[10] 王伟君, 李延华, 张兰威. 光谱方法测定发酵乳中乙醛和双乙酰含量[J].食品科学, 2007,28(11): 460-463.
[11] 王晨, 李晓明, 卢永翎,等. 气相色谱法检测饮料中二羰基化合物[J].食品科学, 2015,36(24): 235-241.
[12] WANG X J, ZHANG H X, LI H, et al. Measurement of α-dicarbonyl compounds in human saliva by pre-column derivatization HPLC[J].Clinical Chemistry and Laboratory Medicine, 2019,57(12): 1 915-1 922.
[13] HURTADO-SANCHEZ M D, ESPINOSA-MANSILLA A, RODRIGUEZ-CACERES M I, et al. Evaluation of liquid chromatographic behavior of lumazinic derivatives, from α-dicarbonyl compounds, in different C18 columns: Application to wine samples using a fused-core column and fluorescence detection[J].Journal of Agricultural and Food Chemistry, 2014,62(1): 97-106.
[14] 郭静, 徐烨, 李琦,等. 分散液相微萃取/高效液相色谱法同时测定啤酒中3种酮类老化物质[J].分析测试学报, 2012,31(11): 1 441-1 445.
[15] 牟翠翠, 冯艳丽, 翟金清,等. 2,4-二硝基苯肼衍生-高效液相色谱测定大气细粒子中二羰基类化合物[J].分析化学, 2010,38(11): 1 573-1 577.
[16] 吴泰钢, 黄才欢, 白卫滨,等. 反相-高效液相色谱法同时检测食品中4种α-二羰基化合物[J].食品工业科技, 2015,36(6): 58-61;65.
[17] 晏印雪, 朱秋劲, 梁美莲,等. 高效液相色谱-荧光检测法测定发酵红肉制品中Neu5GC[J].食品与生物技术学报, 2019,38(5): 66-72.
[18] LI P, ZHU Y, HE S, et al. Development and validation of a high-performance liquid chromatography method for the determination of diacetyl in beer using 4-nitro-o-phenylenediamine as the derivatization reagent[J].Journal of Agricultural and Food Chemistry, 2012,60(12): 3 013-3 019.
[19] 王昕洁, 王继宇, 李恒,等. 3,3′-二氨基联苯胺柱前衍生高效液相色谱法测定酒中双乙酰[J].色谱, 2017,35(8): 837-842.
[20] 陈磊, 栾军, 费晓庆,等. 高效液相色谱法检测新西兰manuka蜂蜜中的甲基乙二醛[J].色谱, 2014,32(2): 189-193.
[21] 相启森, 马云芳, 申瑞玲. 食品中2,3-丁二酮形成机制和检测方法的研究进展[J]. 食品与发酵工业, 2014, 40(11): 208-214.
[22] 崔梅梅. LC-MS/MS检测α-二羰基化合物的方法建立与应用[D].天津:天津科技大学, 2017.
[23] GENSBERGER S, GLOMB M A, PISCHETSRIEDER M. Analysis of sugar degradation products with α-dicarbonyl structure in carbonated soft drinks by UHPLC-DAD-MS/MS[J]. Journal of Agricultural and Food Chemistry, 2013, 61(43): 10 238-10 245.
[24] 董维冲, 郭家良, 李德强,等. 中空纤维离心超滤-HPLC法测定人血浆中阿莫西林的浓度[J]. 中国临床药理学杂志, 2019, 35(14): 1 497-1 500.
[25] 张婷婷, 曾明飞, 金燕,等. 反相高效液相色谱-串联质谱法测定母乳及奶粉中核苷和核苷酸的含量[J]. 分析化学, 2014, 42(4): 585-591.
[26] 金绍明, 宁霄, 曹进. 超高效液相色谱-串联质谱法测定4种饮料中γ-羟基丁酸及其2种相关物质[J]. 食品安全质量检测学报, 2018, 9(19): 5 202-5 207.
[27] 刘彦, 张岩, 付海丽,等. 中空纤维离心超滤法测定乳制品中5种添加剂[J]. 食品研究与开发, 2018, 39(10): 124-128.
[28] 梁晶晶, 沈丹, 徐潇颖,等. 超高效液相色谱-串联质谱法测定动物源性食品中12种青霉素类药物残留量[J]. 食品与发酵工业, 2018, 44(5): 265-269.
[29] LU J J, JIA B J, YANG L, et al. Ultra-high performance liquid chromatography with ultraviolet and tandem mass spectrometry for simultaneous determination of metabolites in purine pathway of rat plasma[J]. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2016, 1 036: 84-92.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司