采用超临界CO2萃取技术,提取维药芹菜籽药用有效成分,用L9(34)正交试验对萃取工艺条件进行了优化。结果表明,超临界萃取芹菜籽挥发油最佳工艺为:萃取温度35℃,萃取压力15MPa,萃取时间90min。在最佳条件下芹菜籽挥发油的得率为7.55%.用GC-MS联用技术鉴定出22种化合物。鉴定出的化合物含量占总挥发油的94.58%,其中主要成分为瑟丹内酯(45.7%)、苯并呋喃酮类(19.72%)、正丁基苯并呋喃酮(8.81%)等药用活性成分。

The supercritical fluid extraction(SFE) for the active volatile compounds in Apium graveolens L.seeds was studied by orthogonal test.The analysis of the volatile compounds in the SFE oil were carried out by the gas chromatography-mass spectrometry method.The optimum conditions were:extraction pressure 15MPa,extraction temperature 35℃,extraction time 90 min.twenty two compounds were found in SFE oil of Apium graveolens L.seeds and main active compounds were Sedanolide(45.70%),Phthalide(19.72%),Butyl phthalide(8.81%).