通过硫酸铵分级沉淀、DEAE-Sepharose Fast Flow阴离子交换柱层析、Sephacryl S-200分子筛柱层析3个步骤,从黑曲霉SL2-111发酵的果胶酶粗品中纯化出一种电泳纯的果胶酯酶。经SDS-PAGE测定其分子质量约为48.8ku。最适pH值和最适温度分别为5.8和60℃。在50℃以下,pH值5.8-6.2之间酶活力相对稳定。

A pectinesterase produced by Aspergillus niger SL2-111 was isolated and purified by three steps including ammonium sulfate precipitation,DEAE Sepharose Fast Flow ion exchang-e and Sephacryl S-200 filtration chromatography.The electrophoretically homogeneous pectineste-rase was obtained with its molecular weight estimated 48.8 ku on SDS-PAGE.Optimal pH and temperature were 5.8 and 60℃,respectively.Its activity maintained relatively stable under such conditions as temperature below 50℃ and pH value between 5.8-6.2.