采用原子吸收分光光度法和高效液相色谱法等技术,检测了甜高粱茎杆汁的主要成分质量浓度,包括蔗糖、果糖和葡萄糖、总氮量以及若干金属元素质量浓度;采取响应面法建立尿素、MgSO4、KH2PO4、CaCl2等的加入量最优组合的二次回归数学模型。结果表明,当尿素、MgSO4、KH2PO4和CaCl2的添加量分别为8.4228、1.0938、4.1702和4.0422g/L时,乙醇发酵得率达93.18%,比优化前乙醇质量浓度提高了9.56g/L。

Atomic absorption spectrophotometry and highly effective liquid phase chromatography techniques were used to detect the main component of the sweet sorghum stem juice,including sucrose,fructose and glucose content,total nitrogen and a number of metal element content;using response surface method to establish the urea,magnesium sulfate,potassium dihydrogen phosphate,calcium chloride the quadratic model of addition,and to gain the mathematic model of optimal combination.The results showed that urea,magnesium sulfate,potassium dihydrogen phosphate,calcium chloride additive amount 8.422 8,1.093 8,4.170 2,and 4.042 2 g/L respectively.So that the alcohol yield reached 93.18%,compared to before optimize fermentation the concentration of alcohol increased 9.56 g/L.