为探讨不同剂量植物油对小鼠肠道微生物、酶活性及血常规的影响,将30只无特定病原体(specific pathogen free,SPF)级昆明小鼠随机分为5组,分别灌胃6.67 mL/(kg·d) (0.1 mL/次)、13.33 mL/(kg·d) (0.2 mL/次)、20.00 mL/(kg·d) (0.3 mL/次)及26.67 mL/(kg·d) (0.4 mL/次)植物油,以0.2 mL蒸馏水/次为正常组,2次/d,共30 d,分析血常规、肠道微生物及酶活性,结果表明,植物油能显著降低小鼠肠道细菌总数和大肠杆菌数(P<0.05)。6.67 mL/(kg·d)组和26.67 mL/(kg·d)组小鼠肠道乳酸菌数显著低于正常组(P<0.05),6.67 mL/(kg·d)组和20.00 mL/(kg·d)组双歧杆菌数较正常组显著降低(P<0.05),而13.33 mL/(kg·d)组双歧杆菌数较正常组显著增加(P<0.05)。6.67 mL/(kg·d)组小鼠的淀粉酶、蛋白酶、木聚糖酶以及纤维素酶活性显著高于正常组(P<0.05),当食用油剂量增加时,4种酶的活性出现不同程度的下降。不同剂量植物油对正常小鼠的红细胞、血小板、血红蛋白以及白细胞等相关指标基本无影响,仅26.67 mL/(kg·d)组的淋巴细胞百分比、中性粒细胞百分比与正常组差异显著(P<0.05)。该文证明适当摄入植物油有助于增加益生菌数,提高肠道消化酶活性。
Intestinal microbiota are important to health. The current research tried to explore the effect of vegetable oil on intestinal microecology, enzyme activity and blood routine. Thirty Specific pathogens free (SPF) Kunming (KM) mice were divided into 5 groups, and given by gavage with 0.1, 0.2, 0.3, 0.4 mL/time vegetable oil and 0.2 mL/time distilled water respectively, twice a day for 30 days. The intestinal microorganism, enzyme activities and blood routines were analyzed. The results showed that vegetable oil could significantly reduce intestinal bacteria and Escherichia coli (P< 0.05). Lactic acid bacteria in 0.1 and 0.4 mL/time group was significantly lower than that in the control group (P< 0.05). Bifidobacteria in 0.1 and 0.3 mL/time group was significantly lower than that in the control group (P<0.05), while Bifidobacteria in 0.2 mL/time group was significantly higher than that in the control group (P<0.05). The activities of amylase, protease, xylanase and cellulase in 0.1 mL/time group were significantly higher than those in the control group (P < 0.05), while the activities of these four enzymes decreased when the dosage of vegetable oil increased. Different doses of vegetable oil had no effect on red blood cells, platelets, hemoglobin and white blood cells. The percentage of lymphocytes and neutrophils in 0.4 mL/time group was significantly different from that in the control group (P< 0.05). In conclusion, proper intake of vegetable oil can increase intestinal probiotics and enzymes activities.
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