该文采用水蒸气蒸馏法对6个不同产地生姜的精油进行提取,并利用气相色谱-质谱联用技术测定其化学组分并进行比较。结果表明,6 个不同产地生姜精油的提取率分别为湖南汝城0.98%、江西吉安1.05%、四川眉山1.08%、山东昌邑0.73%、福建长汀1.13%、云南红河1.27%;分别鉴定出28、28、28、27、29、28种化合物,各占精油总相对百分含量的98.50%、98.14%、98.05%、96.17%、99.03%、97.12%;它们的共有成分有15种,特有成分湖南汝城2种、江西吉安1种、山东昌邑4种、福建长汀1种,而四川眉山和云南红河尚未检测到特有成分。
To investigate the chemical components of essential oil from six different regions of Zingiber officinale, and to compare their differences, the essential oil was extracted from the steam distillation method. The components were identified by GC-MS, and the relative contents of each component was compared by peak area normalization. The results showed that the contents of essential oil from Zingiber officinale of Rucheng Hunan, Ji'an Jiangxi, Meishan Sichuan, Changyi Shangdong, Changting Fujian and Honghe Yunnan were 0.98%, 1.05%, 1.08%, 0.73%, 1.13% and 1.27%, respectively. Among them, 28, 28, 28, 27, 29, 28 compounds were identified, which accounted for 98.50%, 98.14%, 98.05%, 96.17%, 99.03% and 97.12%, respectively. There were 15 kinds of components were shared in all samples, and the unique ones presented in Rucheng Hunan, Ji 'an Jiangxi, Changyi Shandong, Changting Fujian were 2,1,4,1. However, Meishan Sichuan and Honghe Yunnan were not.
[1] 高德民. 中国姜品种遗传多样性的研究[D]. 济南:山东师范大学, 2003.
[2] 吴晓慧, 顾龚平, 张卫明, 等. 姜综合利用及深加工技术研究进展[J]. 中国野生植物资源, 2003, 22(3): 5-9.
[3] 黄静. 姜精油的制备及活血化瘀作用研究[D]. 南宁: 广西医科大学, 2012.
[4] MACIT M S, KOYUNCU S, BETÜL KOCAADAM, et al. Evaluation of ginger (Zingiber Officinale Roscoe) on energy metabolism and obesity: systematic review and meta-analysis[J]. Food Rev Int, 2019(1): 1-22.
[5] ANISHA C, RADHAKRISHNAN E K. Metabolite analysis of endophytic fungi from cultivars of Zingiber officinale Rosc. identifies myriad of bioactive compounds including tyrosol[J]. Biotech, 2017, 7(2): 146-155.
[6] BERISTAIN-BAUZA S D C, HERNANDEZ-CARRÁNZA P, CID-PÉREZ T S, et al. Antimicrobial activity of ginger(Zingiber Officinale) and its application in food products[J]. Food Reviews International,, 2019,41:1-20.
[7] 刘鑫, 张宏伟, 傅若秋, 等. 生姜中姜酚类活性成分的抗肿瘤作用及其机制[J]. 第三军医大学学报, 2017, 39 (9): 884-890.
[8] SHAHEDUR, RASIF I, FAIZUS S. Retracted article: In vitro antioxidant and anticancer activity of young Zingiber officinale against human breast carcinoma cell lines [J]. BMC Complementary & Alternative Medicine, 2012, 12: 206-206.
[9] OBOH G, AKINYEMI A J, ADEMILUYI A O. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe2+induced lipid peroxidation in rat brain in vitro[J]. Experimental & Toxicologic Pathology, 2012, 64(1-2): 31-36.
[10] HOSSEINZADEH A, BAHRAMPOUR JUYBARI K, FATEMI M J, et al. Protective effect of ginger(Zingiber officinale Roscoe) extract against oxidative stress and mitochondrial apoptosis induced by interleukin-1β in cultured chondrocytes [J]. Cells Tissues Organs, 2017, 204(5-6): 241-250.
[11] LIANG N, SANG Y, LIU W, et al. Anti-inflammatory effects of gingerol on lipopolysaccharide-stimulated RAW264.7 cells by inhibiting NF-kB singnaling pathway [J]. Inflammation, 2018,41 (5): 835-845.
[12] FUNK J L, FRYE J B, OYARZO J N, et al. Anti-inflammatory effects of the essential oils of ginger(Zingiber officinale Roscoe) in experimental rheumatoid arthritis[J]. Pharmanutrition,2016,4(3): 123-131.
[13] 徐宗季, 鞠兴荣, 袁建, 等. 生姜精油挥发性物质的主成分分析[J]. 粮食与食品工业, 2016, 23(4): 5-10.
[14] FENG J, DU Z, ZHANG L, et al. Chemical composition and skin protective effects of essential oil obtained from ginger (Zingiber officinale Roscoe) [J]. J Essen Oil Bear PL, 2018, 21(6): 1 542-1 549.
[15] 王菲, 黄玉艾, 沈雪, 等. 不同生姜提取物的急性毒性研究[J]. 食品研究与开发, 2009, 30(1): 138-140.
[16] ALI B H, BLUNDEN G, TANIRA M O, et al. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): A review of recent research[J]. Food and Chemical Toxicology, 2008, 46(2): 409-420.
[17] AWAM U A, ALI S, SHAHNAWAZ A M, et al. Biological activities of Allium sativum and Zingiber officinale extracts on clinically important bacterial pathogens, their phytochemical and FT-IR spectroscopic analysis [J]. Pakistan Journal of Pharmaceutical ences, 2017, 30(3): 729-745.
[18] 付爱华, 尹建元, 孙莹, 等. 黄精和生姜抗皮肤癣菌活性研究[J]. 白求恩医科大学学报, 2001, 27(4): 384-385.
[19] MILLAR J G. Rapid and simple isolation of zingiberene from ginger essential oil[J]. Journal of Natural Products, 1998, 61(8): 1 025-1 026.
[20] 庞亚如, 胡智慧, 肖冬光, 等. 柠檬烯和红没药烯的微生物代谢工程[J]. 生物工程学报, 2018,34(1): 24-33.
[21] 鹿浩志, 刘敏轩, 田亚亚, 等. 生姜精油的提取及 GC- MS分析[J]. 食品工业科技, 2019, 40(14): 208-213.
[22] 徐宗季, 鞠兴荣, 袁建, 等. 生姜精油挥发性物质的主成分分析[J]. 粮食与食品工业, 2016, 23(4): 5-10.
